Western Blot (WB)
(Western Blot Analysis: Representative lot data. U2OS cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Upf1 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Upf1 (~125kD).)
Rabbit anti-Human RENT1 Polyclonal Antibody | anti-RENT1 antibody
RENT1 (Regulator of Nonsense Transcripts 1, ATP-dependent Helicase RENT1, Delta Helicase, FLJ43809, FLJ46894, hUPF1, KIAA0221, Nonsense mRNA Reducing Factor 1, NORF1, pNORF1, pNORF-1, UP Frameshift 1, UPF1, Up Frameshift Mutation 1 Homolog (S. cerevisiae)
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blot Analysis: Representative lot data. U2OS cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Upf1 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Upf1 (~125kD).)
Western Blot (WB)
(Western Blot Analysis: Representative lot data. U2OS cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Upf1 (0.5ug/ml). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Upf1 (~125kD).)
NCBI and Uniprot Product Information
Uniprot Description
RENT1: RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1- eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability. Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Associates with the SGM1C complex; is phosphorylated by the complex kinase component SGM1. Interacts with UPF2, UPF3A and UPF3B. Interacts with EST1A and SLBP. Interacts (when hyperphosphorylated) with PNRC2. Interacts with EIF2C1, EIF2C2 and GSPT2. Ubiquitous. Belongs to the DNA2/NAM7 helicase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; C2H2-type zinc finger protein; EC 3.6.4.-; Hydrolase; EC 3.6.1.-
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus; chromatin
Molecular Function: protein binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; helicase activity; ATP binding; ATP-dependent RNA helicase activity
Biological Process: mRNA export from nucleus; dosage compensation, by inactivation of X chromosome; mRNA catabolic process, nonsense-mediated decay; gene expression; DNA replication; DNA repair; regulation of translational termination
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Product Notes
The RENT1 upf1 (Catalog #AAA6497198) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RENT1 (Regulator of Nonsense Transcripts 1, ATP-dependent Helicase RENT1, Delta Helicase, FLJ43809, FLJ46894, hUPF1, KIAA0221, Nonsense mRNA Reducing Factor 1, NORF1, pNORF1, pNORF-1, UP Frameshift 1, UPF1, Up Frameshift Mutation 1 Homolog (S. cerevisiae) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RENT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 0.5-2ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RENT1 upf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RENT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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