Western Blot (WB)
(Western Blot analysis of IQWD1 expression in transfected 293T cell line by IQWD1 polyclonal antibody. Lane 1: IQWD1 transfected lysate (56.43kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human IQWD1 Polyclonal Antibody | anti-DCAF6 antibody
IQWD1 (DCAF6, DDB1- and CUL4-associated Factor 6, Androgen Receptor Complex-associated Protein, ARCAP, IQ Motif and WD Repeat-containing Protein 1, Nuclear Receptor Interaction Protein, NRIP, MSTP055)
Gene Names
DCAF6; NRIP; ARCAP; IQWD1; PC326; MSTP055; RP4-745I14.1; 1200006M05Rik
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
IQWD1; Polyclonal Antibody; IQWD1 (DCAF6; DDB1- and CUL4-associated Factor 6; Androgen Receptor Complex-associated Protein; ARCAP; IQ Motif and WD Repeat-containing Protein 1; Nuclear Receptor Interaction Protein; NRIP; MSTP055); Anti -IQWD1 (DCAF6; anti-DCAF6 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IQWD1.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MLSRWFEEASEVAQSNRGRGRSRPRGGTSQSDISTLPTVPSSPDLEVSETAMEVDTPAEQFLQPSTSSTMSAQAHSTSSPTESPHSTPLLSSPDSEQRQSVEASGHHTHHQSDNNNEKLSPKPGTGEPVLSLHYSTEGTTTSTIKLNFTDEWSSIASSSRGIGSHCKSEGQEESFVPQSSVQPPEGDSETKAPEESSEDATKYQEGVSAENPVENHINITQSDKFTAKPLDSNSGERNDLNLDRSCGVPEESASSEKAKEPETSDQTSTESATNENNTNPEPQFQTEATGPSAHEETSTRDSALQDTDDSDDDPVLIPGARYRAGPGDRRSAVARIQEFFRRRKERKEMEELDTLNIRRPLVKMVYKGHRNSRTMIKEANFWGANFVMSGSDCGHIFIWDRHTAEHLMLLEADNHVVNCLQPHPFDPILASSGIDYDIKIWSPLEESRIFNRKLADEVITRNELMLEETRNTITVPASFMLRMLASLNHIRADRLEGDRSEGSGQENENEDEE
Applicable Applications for anti-DCAF6 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length human IQWD1, aa1-513 (AAH25262.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of IQWD1 expression in transfected 293T cell line by IQWD1 polyclonal antibody. Lane 1: IQWD1 transfected lysate (56.43kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of IQWD1 expression in transfected 293T cell line by IQWD1 polyclonal antibody. Lane 1: IQWD1 transfected lysate (56.43kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to IQWD1 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to IQWD1 on HeLa cell. [antibody concentration 10ug/ml].)
Related Product Information for anti-DCAF6 antibody
Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Product Categories/Family for anti-DCAF6 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
96,292 Da
NCBI Official Full Name
IQWD1 protein
NCBI Official Synonym Full Names
DDB1 and CUL4 associated factor 6
NCBI Official Symbol
DCAF6
NCBI Official Synonym Symbols
NRIP; ARCAP; IQWD1; PC326; MSTP055; RP4-745I14.1; 1200006M05Rik
NCBI Protein Information
DDB1- and CUL4-associated factor 6; IQ motif and WD repeats 1; nuclear receptor interaction protein; IQ motif and WD repeat-containing protein 1; androgen receptor complex-associated protein
UniProt Protein Name
DDB1- and CUL4-associated factor 6
Protein Family
UniProt Gene Name
DCAF6
UniProt Synonym Gene Names
IQWD1; ARCAP; NRIP
UniProt Entry Name
DCAF6_HUMAN
Uniprot Description
Function: Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Ref.1 Ref.9
Pathway: Protein modification; protein ubiquitination.
Subunit structure: Interacts with the nuclear receptors NR3C1 and AR in the presence of ligand. Interacts with DDB1, CUL4A and CUL4B. Ref.1 Ref.9
Subcellular location: Nucleus Ref.1.
Tissue specificity: Highly expressed in skeletal muscle and testis. Expressed to a lesser degree in heart, prostate, and adrenal gland. Ref.1
Sequence similarities: Contains 1 IQ domain.Contains 7 WD repeats.
Research Articles on DCAF6
Similar Products
Product Notes
The DCAF6 dcaf6 (Catalog #AAA649540) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IQWD1 (DCAF6, DDB1- and CUL4-associated Factor 6, Androgen Receptor Complex-associated Protein, ARCAP, IQ Motif and WD Repeat-containing Protein 1, Nuclear Receptor Interaction Protein, NRIP, MSTP055) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IQWD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the DCAF6 dcaf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLSRWFEEAS EVAQSNRGRG RSRPRGGTSQ SDISTLPTVP SSPDLEVSET AMEVDTPAEQ FLQPSTSSTM SAQAHSTSSP TESPHSTPLL SSPDSEQRQS VEASGHHTHH QSDNNNEKLS PKPGTGEPVL SLHYSTEGTT TSTIKLNFTD EWSSIASSSR GIGSHCKSEG QEESFVPQSS VQPPEGDSET KAPEESSEDA TKYQEGVSAE NPVENHINIT QSDKFTAKPL DSNSGERNDL NLDRSCGVPE ESASSEKAKE PETSDQTSTE SATNENNTNP EPQFQTEATG PSAHEETSTR DSALQDTDDS DDDPVLIPGA RYRAGPGDRR SAVARIQEFF RRRKERKEME ELDTLNIRRP LVKMVYKGHR NSRTMIKEAN FWGANFVMSG SDCGHIFIWD RHTAEHLMLL EADNHVVNCL QPHPFDPILA SSGIDYDIKI WSPLEESRIF NRKLADEVIT RNELMLEETR NTITVPASFM LRMLASLNHI RADRLEGDRS EGSGQENENE DEE. It is sometimes possible for the material contained within the vial of "IQWD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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