Rabbit anti-Human, Mouse Phosphorylase Kinase Subunit gamma 2 Polyclonal Antibody | anti-PHKG2 antibody

Phosphorylase Kinase Subunit gamma 2, NT (PHKG2, Phosphorylase B Kinase gamma Catalytic Chain Testis/liver Isoform, PHK-gamma-T, GSD9C, PSK-C3) (APC)

Cat. Num. AAA6493333

• Gene Names: PHKG2; GSD9C
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Phosphorylase Kinase Subunit gamma 2; Polyclonal Antibody; NT (PHKG2; Phosphorylase B Kinase gamma Catalytic Chain Testis/liver Isoform; PHK-gamma-T; GSD9C; PSK-C3) (APC); Phosphorylase Kinase; gamma-2 (Testis); EC=2.7.11.19; anti-PHKG2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PHKG2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PHKG2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PHKG2 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-PHKG2 Pab is used in Western blot to detect PHKG2 in mouse kidney tissue lysate.)

Western Blot (WB)

(Western blot analysis of PHKG2 (arrow) using PHKG2 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHKG2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(PHKG2 Antibody (E56) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PHKG2 antibody detected the PHKG2 protein (arrow).)

Product Categories/Family for anti-PHKG2 antibody

Antibodies; Protein Kinases

References

1. Burwinkel, B., et al., Hum. Mol. Genet. 7(1):149-154 (1998). 2. Maichele, A.J., et al., Nat. Genet. 14(3):337-340 (1996). 3. Whitmore, S.A., et al., Genomics 20(2):169-175 (1994). 4. Hanks, S.K., Mol. Endocrinol. 3(1):110-116 (1989). 5. Hanks, S.K., Proc. Natl. Acad. Sci. USA 84(2):388-392 (1987).

NCBI and Uniprot Product Information

• NCBI GI #: 4505785
• NCBI GeneID: 5261
• NCBI Accession #: NP_000285
• NCBI GenBank Nucleotide #: NM_000294
• UniProt Accession #: P15735; P11800; A8K0C7; B4DEB7; E9PEU3
• Molecular Weight: 43,158 Da
• NCBI Official Full Name: phosphorylase b kinase gamma catalytic chain, liver/testis isoform isoform 1
• NCBI Official Synonym Full Names: phosphorylase kinase, gamma 2 (testis)
• NCBI Official Symbol: PHKG2
• NCBI Official Synonym Symbols: GSD9C
• NCBI Protein Information: phosphorylase b kinase gamma catalytic chain, liver/testis isoform; PHK-gamma-LT; PHK-gamma-T; PSK-C3; Phosphorylase kinase, gamma 2 (testis/liver); phosphorylase b kinase gamma catalytic chain, testis/liver isoform; phosphorylase kinase subunit gamma-2
• UniProt Protein Name: Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
• Protein Family: Phosphorylase b kinase
• UniProt Gene Name: PHKG2
• UniProt Synonym Gene Names: PHK-gamma-LT; PHK-gamma-T
• UniProt Entry Name: PHKG2_HUMAN