Rabbit anti-Human VPS25 Polyclonal Antibody | anti-VPS25 antibody

VPS25, NT (Vacuolar Protein-sorting-associated Protein 25, hVps25, Dermal Papilla-derived Protein 9, ELL-associated Protein of 20kD, ESCRT-II Complex Subunit VPS25, DERP9, EAP20)

Cat. Num. AAA649234

• Gene Names: VPS25; DERP9; EAP20; FAP20
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: VPS25; Polyclonal Antibody; NT (Vacuolar Protein-sorting-associated Protein 25; hVps25; Dermal Papilla-derived Protein 9; ELL-associated Protein of 20kD; ESCRT-II Complex Subunit VPS25; DERP9; EAP20); Anti -VPS25; anti-VPS25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human VPS25.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.01% sodium azide, 50% glycerol.

• Applicable Applications for anti-VPS25 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
• Immunogen: Synthetic peptide corresponding to human VPS25 at N-terminal (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with VPS25 antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-VPS25 antibody

VPS25 is a component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL.

Product Categories/Family for anti-VPS25 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 14150155
• NCBI GeneID: 84313
• NCBI Accession #: NP_115729.1
• NCBI GenBank Nucleotide #: NM_032353.3
• UniProt Accession #: Q9BRG1; B2R581
• Molecular Weight: 20,748 Da
• NCBI Official Full Name: vacuolar protein-sorting-associated protein 25
• NCBI Official Synonym Full Names: vacuolar protein sorting 25 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS25
• NCBI Official Synonym Symbols: DERP9; EAP20; FAP20
• NCBI Protein Information: vacuolar protein-sorting-associated protein 25; ESCRT-II complex subunit VPS25; ELL-associated protein of 20 kDa; dermal papilla-derived protein 9
• UniProt Protein Name: Vacuolar protein-sorting-associated protein 25
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS25
• UniProt Synonym Gene Names: DERP9; EAP20; hVps25
• UniProt Entry Name: VPS25_HUMAN

NCBI Description

This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A pseudogene of this gene is present on chromosome 1. [provided by RefSeq, Jul 2013]

Uniprot Description

Function: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. Ref.12

Subunit structure: Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45

By similarity. Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and 2 copies of VPS25. Interacts with CFTR; the interaction requires misfolded CFTR. Interacts (via C-terminal half) with the ESCRT-III subunit CHMP6 (via N-terminal half). Ref.5 Ref.6 Ref.8 Ref.9 Ref.11

Subcellular location: Cytoplasm. Endosome membrane. Nucleus › nucleoplasm. Note: Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6. Ref.8 Ref.11

Tissue specificity: Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung. Ref.10

Sequence similarities: Belongs to the VPS25 family.