Rabbit anti-Human PARK2 Polyclonal Antibody | anti-PARK2 antibody

PARK2, CT (E3 Ubiquitin-protein Ligase Parkin, PRKN, Parkinson Juvenile Disease Protein 2, Parkinson Disease Protein 2) (APC)

Cat. Num. AAA6492105

• Gene Names: PARK2; PDJ; PRKN; AR-JP; LPRS2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: PARK2; Polyclonal Antibody; CT (E3 Ubiquitin-protein Ligase Parkin; PRKN; Parkinson Juvenile Disease Protein 2; Parkinson Disease Protein 2) (APC); anti-PARK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Park2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PARK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide in the N-terminal region of human Park2 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Park2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Park2 antibody detected the Park2 protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Parkin Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Parkin Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(The anti-Parkin (C-term) Pab is used in Western blot to detect Parkin in mouse kidney tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PARK2 antibody

Antibodies; Ubiquitin Ligases

References

1. Kumru, H., et al., Ann. Neurol. 56(4):599-603 (2004). 2. Pigullo, S., et al., Parkinsonism Relat. Disord. 10(6):357-362 (2004). 3. Yao, D., et al., Proc. Natl. Acad. Sci. USA 101(29):10810-10814 (2004). 4. West, A.B., et al., J. Biol. Chem. 279(28):28896-28902 (2004). 5. Wang, F., et al., Genes Chromosomes Cancer 40(2):85-96 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4758884
• NCBI GeneID: 5071
• NCBI Accession #: NP_004553.1; NP_054642.1; NP_054643.1
• NCBI GenBank Nucleotide #: NM_004562.1
• Molecular Weight: 46,413 Da
• NCBI Official Full Name: parkin isoform 1
• NCBI Official Synonym Full Names: parkin RBR E3 ubiquitin protein ligase
• NCBI Official Symbol: PARK2
• NCBI Official Synonym Symbols: PDJ; PRKN; AR-JP; LPRS2
• NCBI Protein Information: E3 ubiquitin-protein ligase parkin; parkinson juvenile disease protein 2; parkinson protein 2, E3 ubiquitin protein ligase (parkin); Parkinson disease (autosomal recessive, juvenile) 2, parkin
• Protein Family: E3 ubiquitin-protein ligase