Rabbit anti-Mouse, Rat p67 phox Polyclonal Antibody | anti-NCF2 antibody

p67 phox (NCF2, Neutrophil Cytosol Factor 2, 67kD Neutrophil Oxidase Factor, NCF-2, Neutrophil NADPH Oxidase Factor 2, NOXA2) (AP)

Cat. Num. AAA6491416

• Gene Names: NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: p67 phox; Polyclonal Antibody; p67 phox (NCF2; Neutrophil Cytosol Factor 2; 67kD Neutrophil Oxidase Factor; NCF-2; Neutrophil NADPH Oxidase Factor 2; NOXA2) (AP); anti-NCF2 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse p67-phox at ~67kD. Species Crossreactivity: rat
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-NCF2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 0.5-2.0ug/ml detects p67-phox in RIPA lysates from RAW 264.7 cells.; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptides corresponding to the first 15aa from the N-terminal and the last 15aa on the C-terminal of mouse p67-phox, conjugated to KLH.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(RAW 264.7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS611006 (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates p67-phox (67kD).)

Immunohistochemistry (IHC)

(Immunohistochemistry: Slices from perfused rat brains (0.9% saline and 10% formalin; 40um slices) were used for immunohistochemical staining with MBS611006 (1:2000 incubated for 24 hr). Detection was with a Tyramide Amplification kit. The image shown is of labeled cells in the cortex of a normal male rat brain (magnification is 20X). Data generously provided by Ken Brittian, University of Louisville.)

Product Categories/Family for anti-NCF2 antibody

Antibodies; Enzymes; Oxidase

References

1. Mizuki, K., et al., Eur. J. Biochem. 251: 573-582 (1998). 2. Okamura, N., et al., J. Clin. Invest. 85: 1583-1587 (1990). 3. Ellson, C., et al., EMBO J. 25: 4468-4478 (2006). 4. Vignais, P.V., et al., Cell. Mol. Life Sci. 59: 1428-1459 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 67189970
• NCBI GeneID: 4688
• NCBI Accession #: NP_000424
• NCBI GenBank Nucleotide #: NM_000433
• UniProt Accession #: P19878
• Molecular Weight: 60kDa
• NCBI Official Full Name: neutrophil cytosol factor 2 isoform 1
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 2
• NCBI Official Symbol: NCF2
• NCBI Official Synonym Symbols: NCF-2; NOXA2; P67PHOX; P67-PHOX
• NCBI Protein Information: neutrophil cytosol factor 2
• UniProt Protein Name: Neutrophil cytosol factor 2
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF2
• UniProt Synonym Gene Names: NOXA2; P67PHOX; NCF-2
• UniProt Entry Name: NCF2_HUMAN

NCBI Description

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: cytoplasm; nucleolus; acrosome; cytosol; NADPH oxidase complex

Molecular Function: protein C-terminus binding; protein binding; electron carrier activity; Rac GTPase binding

Biological Process: respiratory burst; response to drug; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to lipopolysaccharide; cellular response to hormone stimulus; antigen processing and presentation of peptide antigen via MHC class I; response to hyperoxia; positive regulation of neuron apoptosis; response to glucose stimulus; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; cellular defense response; response to activity; response to progesterone stimulus; vascular endothelial growth factor receptor signaling pathway; superoxide release; positive regulation of blood pressure; aging

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii