Rabbit anti-Mouse LRWD1 Polyclonal Antibody | anti-LRWD1 antibody

LRWD1, NT (LRWD1, ORCA, Leucine-rich repeat and WD repeat-containing protein 1, ORC-associated protein, Origin recognition complex-associated protein)

Cat. Num. AAA649125

• Gene Names: LRWD1; ORCA; CENP-33
• Reactivity: Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: LRWD1; Polyclonal Antibody; NT (LRWD1; ORCA; Leucine-rich repeat and WD repeat-containing protein 1; ORC-associated protein; Origin recognition complex-associated protein); Anti -LRWD1; anti-LRWD1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-LRWD1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: LRWD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-33 amino acids from the N-terminal region of human LRWD1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LRWD1 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LRWD1 antibody detected the LRWD1 protein (arrow).)

Immunohistochemistry (IHC)

(LRWD1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRWD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-LRWD1 antibody

LRWD1, Leucine rich repeats and WD repeat domain containing 1, contains 3 LRR (leucine-rich) repeats and 5 WD repeats.

Product Categories/Family for anti-LRWD1 antibody

Antibodies; Abs to WD Repeat Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 23097240
• NCBI GeneID: 222229
• NCBI Accession #: NP_690852.1
• NCBI GenBank Nucleotide #: NM_152892.1
• UniProt Accession #: Q9UFC0; Q8N0T9; Q8WV43; Q96GJ2; A8K4K2; B2R9G2
• Molecular Weight: 70,861 Da
• NCBI Official Full Name: leucine-rich repeat and WD repeat-containing protein 1
• NCBI Official Synonym Full Names: leucine-rich repeats and WD repeat domain containing 1
• NCBI Official Symbol: LRWD1
• NCBI Official Synonym Symbols: ORCA; CENP-33
• NCBI Protein Information: leucine-rich repeat and WD repeat-containing protein 1; centromere protein 33; ORC-associated protein; origin recognition complex associated; origin recognition complex-associated protein
• UniProt Protein Name: Leucine-rich repeat and WD repeat-containing protein 1
• Protein Family: Leucine-rich repeat and WD repeat-containing protein
• UniProt Gene Name: LRWD1
• UniProt Synonym Gene Names: CENP33; ORCA; CENP-33; ORC-associated protein; ORCA
• UniProt Entry Name: LRWD1_HUMAN

Uniprot Description

Function: Required for G1/S transition. Recruits and stabilizes the origin recognition complex (ORC) onto chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3 in a cooperative manner with DNA methylation. Required for silencing of major satellite repeats. May be important ORC2, ORC3 and ORC4 stability. Ref.12 Ref.13 Ref.14 Ref.18 Ref.19

Subunit structure: Integral component of the ORC complex. Directly interacts with CDT1, GMNN and ORC2. Interacts with ORC2 only when non-ubiquitinated; this interaction prevents LRWD1 ubiquitination and degradation. Some of these interactions are regulated in a cell-cycle dependent manner. Interaction with ORC1 occurs predominantly during G1. Association with phosphorylated ORC1 during mitosis is not efficient. Interaction with CDT1 occurs during G1 phase, as well as during mitosis with phosphorylated CDT1. Interaction with GMNN occurs from G1/S to mitosis. Interaction with ORC2 is observed throughout the cell cycle. The stoichiometry of the ORCA/ORC/CDT1/GMNN complex is 1:1:1:2. Interacts with CUL4A and DDB1; this interaction may lead to ubiquitination. Ref.12 Ref.13 Ref.14 Ref.17 Ref.19

Subcellular location: Nucleus. Chromosome › centromere. Chromosome › telomere. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

By similarity. Note: Localizes to heterochromatin during G1 phase. Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres. Recruitment to pericentric heterochromatin largely depends on the presence of H3K9me3. Ref.13 Ref.14 Ref.17 Ref.18

Tissue specificity: Testis-specific. Drastically down-regulated in testis from patients with Sertoli cell-only syndrome (SCOS). Ref.7

Developmental stage: Regulated in a cell-cycle dependent manner. Highest expression in G1 phase. Expression decreases during S phase, rises again during G2 and drops during mitosis (at protein level). In contrast to protein levels, transcript levels do not show any significant variation during different stages of the cell cycle (Ref.17). Ref.14 Ref.17

Domain: The entire WD repeat region is required for the interaction with ORC, CDT1 and GMNN, as well as for association with chromatin and for binding to histone H3 and H4 trimethylation marks H3K9me3 and H4K20me3. Ref.14 Ref.18 Ref.19

Post-translational modification: Ubiquitinated; undergoes 'Lys-48'-linked polyubiquitination leading to proteasomal degradation. Ubiquitination occurs within the WD repeats at the end of the G1 phase. Ubiquitination may be catalyzed by the CUL4-DDB1 E3 ubiquitin-protein ligase complex and other E3 ligases. Ref.17

Sequence similarities: Belongs to the LRWD1 family.Contains 4 LRR (leucine-rich) repeats.Contains 5 WD repeats.