Western Blot (WB)
(WB: Mouse testes homogenate probed with MBS614929 at 2ug/ml.)
Rabbit anti-Mouse Nck beta Polyclonal Antibody
Nck beta (MaxLight 750)
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Nck beta; Polyclonal Antibody; Nck beta (MaxLight 750); anti-Nck beta antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Nck beta, Mr 47kD. Species Sequence Homology: human
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-Nck beta antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 1:100. Immunoprecipitates
Applications are based on unconjugated antibody.
WB: 0.5-2ug/ml. Detects Nck beta in RIPA lysates of murine testes and spleen homogenates. Mouse testes homogenate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Applications are based on unconjugated antibody.
WB: 0.5-2ug/ml. Detects Nck beta in RIPA lysates of murine testes and spleen homogenates. Mouse testes homogenate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunogen
Synthetic peptide corresponding to aa175-190, EAPSFLSLRRGTALSN, from mouse Nck beta, conjugated to KLH.
Conjugate
MaxLight750
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(WB: Mouse testes homogenate probed with MBS614929 at 2ug/ml.)
Western Blot (WB)
(WB: Mouse testes homogenate probed with MBS614929 at 2ug/ml.)
Product Categories/Family for anti-Nck beta antibody
References
1. Ling, P., et al., J. Biol. Chem. 276: 18, 908-18, 914 (2001).
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Product Notes
The Nck beta (Catalog #AAA6489493) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nck beta (MaxLight 750) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nck beta can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). IP: 1:100. Immunoprecipitates Applications are based on unconjugated antibody. WB: 0.5-2ug/ml. Detects Nck beta in RIPA lysates of murine testes and spleen homogenates. Mouse testes homogenate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Researchers should empirically determine the suitability of the Nck beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nck beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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