Rabbit anti-Human SHCBP1 Polyclonal Antibody | anti-SHCBP1 antibody

SHCBP1, CT (SHCBP1, SHC SH2 domain-binding protein 1)

Cat. Num. AAA648911

• Gene Names: SHCBP1; PAL
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SHCBP1; Polyclonal Antibody; CT (SHCBP1; SHC SH2 domain-binding protein 1); Anti -SHCBP1; anti-SHCBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SHCBP1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: SHCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 509-538 amino acids from the C-terminal region of human SHCBP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SHCBP1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SHCBP1 antibody detected the SHCBP1 protein (arrow).)

Immunohistochemistry (IHC)

(SHCBP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SHCBP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SHCBP1 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SHCBP1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 12654279
• NCBI GeneID: 79801
• UniProt Accession #: Q8NEM2; Q96N60; Q9BVS0; Q9H6P6
• Molecular Weight: 75,690 Da
• NCBI Official Full Name: SHCBP1 protein, partial
• NCBI Official Synonym Full Names: SHC SH2-domain binding protein 1
• NCBI Official Symbol: SHCBP1
• NCBI Official Synonym Symbols: PAL
• NCBI Protein Information: SHC SH2 domain-binding protein 1
• UniProt Protein Name: SHC SH2 domain-binding protein 1
• Protein Family: SHC SH2 domain-binding protein
• UniProt Gene Name: SHCBP1
• UniProt Entry Name: SHCBP_HUMAN

Uniprot Description

Function: May play a role in signaling pathways governing cellular proliferation, cell growth and differentiation. May be a component of a novel signaling pathway downstream of Shc. Acts as a positive regulator of FGF signaling in neural progenitor cells

By similarity.

Subunit structure: Interacts directly with isoform p52shc of SHC1 via its SH2 domain. Interacts with TRIM71; leading to enhance SHCBP1 protein stability

By similarity.

Sequence similarities: Contains 5 PbH1 repeats.

Sequence caution: The sequence BAB15208.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.