Rabbit anti-Human SHCBP1 Polyclonal Antibody | anti-SHCBP1 antibody

SHCBP1, CT (SHCBP1, SHC SH2 domain-binding protein 1) (FITC)

Cat. Num. AAA6347387

• Gene Names: SHCBP1; PAL
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SHCBP1; Polyclonal Antibody; CT (SHCBP1; SHC SH2 domain-binding protein 1) (FITC); SHC SH2 domain-binding protein 1; anti-SHCBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-SHCBP1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SHCBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 509-538 amino acids from the C-terminal region of human SHCBP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SHCBP1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the SHCBP1 antibody detected the SHCBP1 protein (arrow).)

Immunohistochemistry (IHC)

(SHCBP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SHCBP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SHCBP1 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-SHCBP1 antibody

Antibodies; Binding Proteins

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Obuse, C., et al. Genes Cells 9(2):105-120(2004). Schmandt, R., et al. Oncogene 18(10):1867-1879(1999). Robertson, N.G., et al. Genomics 23(1):42-50(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 190358504
• NCBI GeneID: 79801
• NCBI Accession #: NP_079021.3
• NCBI GenBank Nucleotide #: NM_024745.4
• UniProt Accession #: Q8NEM2; Q96N60; Q9BVS0; Q9H6P6
• Molecular Weight: 75,690 Da
• NCBI Official Full Name: SHC SH2 domain-binding protein 1
• NCBI Official Synonym Full Names: SHC SH2-domain binding protein 1
• NCBI Official Symbol: SHCBP1
• NCBI Official Synonym Symbols: PAL
• NCBI Protein Information: SHC SH2 domain-binding protein 1
• UniProt Protein Name: SHC SH2 domain-binding protein 1
• Protein Family: SHC SH2 domain-binding protein
• UniProt Gene Name: SHCBP1
• UniProt Entry Name: SHCBP_HUMAN

Uniprot Description

Function: May play a role in signaling pathways governing cellular proliferation, cell growth and differentiation. May be a component of a novel signaling pathway downstream of Shc. Acts as a positive regulator of FGF signaling in neural progenitor cells

By similarity.

Subunit structure: Interacts directly with isoform p52shc of SHC1 via its SH2 domain. Interacts with TRIM71; leading to enhance SHCBP1 protein stability

By similarity.

Sequence similarities: Contains 5 PbH1 repeats.

Sequence caution: The sequence BAB15208.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.