Rabbit anti-Human MYC Polyclonal Antibody | anti-MYC antibody

MYC (T58) (Myc Proto-oncogene Protein, c-Myc, Proto-oncogene c-Myc, Transcription Factor p64, Class E Basic Helix-loop-helix Protein 39, bHLHe39) (FITC)

Cat. Num. AAA6488778

• Gene Names: MYC; MRTL; MYCC; c-Myc; bHLHe39
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MYC; Polyclonal Antibody; MYC (T58) (Myc Proto-oncogene Protein; c-Myc; Proto-oncogene c-Myc; Transcription Factor p64; Class E Basic Helix-loop-helix Protein 39; bHLHe39) (FITC); anti-MYC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MYC.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MYC antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding T58 of human MYC (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MYC (arrow) using rabbit polyclonal MYC Antibody (T58). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MYC gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MYC Antibody (T58), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-MYC antibody

Antibodies; Cancer Markers

References

1. Lima, F.P., Am. J. Clin. Pathol. 129(5), 723-726 (2008). 2. Ida, C., Biosci. Biotechnol. Biochem. 72(3), 868-871 (2008). 3. Iijima, S., Eur. J. Biochem. 206(2), 595-603 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 71774083
• NCBI GeneID: 4609
• NCBI Accession #: NP_002458
• NCBI GenBank Nucleotide #: NM_002467.5
• UniProt Accession #: P01106; P01107; Q14026; A8WFE7
• Molecular Weight: 50,565 Da
• NCBI Official Full Name: myc proto-oncogene protein isoform 1
• NCBI Official Synonym Full Names: MYC proto-oncogene, bHLH transcription factor
• NCBI Official Symbol: MYC
• NCBI Official Synonym Symbols: MRTL; MYCC; c-Myc; bHLHe39
• NCBI Protein Information: myc proto-oncogene protein
• UniProt Protein Name: Myc proto-oncogene protein
• Protein Family: Mycosin
• UniProt Gene Name: MYC
• UniProt Synonym Gene Names: BHLHE39; bHLHe39
• UniProt Entry Name: MYC_HUMAN

NCBI Description

This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]

Uniprot Description

Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.

Protein type: DNA-binding; Transcription factor; Oncoprotein; Nucleolus

Chromosomal Location of Human Ortholog: 8q24.21

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus; cytosol

Molecular Function: protein dimerization activity; protein binding; DNA binding; protein complex binding; transcription factor activity; transcription factor binding

Biological Process: oxygen transport; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; chromosome organization and biogenesis; positive regulation of fibroblast proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to gamma radiation; cell cycle arrest; response to drug; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; regulation of telomere maintenance; MAPKKK cascade; negative regulation of cell division; negative regulation of stress-activated MAPK cascade; negative regulation of monocyte differentiation; chromatin remodeling; ureteric bud branching; negative regulation of fibroblast proliferation; regulation of gene expression; energy reserve metabolic process; positive regulation of transcription from RNA polymerase II promoter; gene expression; response to DNA damage stimulus; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

Disease: Burkitt Lymphoma