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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALOX15 protein (Right) or empty vector (Left) detected with ALOX15 antibody)

Mouse ALOX15 Monoclonal Antibody | anti-ALOX15 antibody

ALOX15 antibody

Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
ALOX15 antibody was purified by affinity chromatography.
Synonyms
ALOX15; Monoclonal Antibody; ALOX15 antibody; Monoclonal ALOX15; Anti-ALOX15; 15-LOX-1; 15LOX-1; ALOX 15; ALOX-15; anti-ALOX15 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7H6
Purity/Purification
ALOX15 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Applicable Applications for anti-ALOX15 antibody
Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
IHC: 1:150
WB: 1:500-2000
Biological Significance
Lipoxygenases (LOXs) are a family of non-heme iron dioxygenases that are involved in the production and metabolism of fatty acid hydroperoxidases. There are six lipoxygenase isozymes; 5-LOX, 12R-LOX, 12S-LOX, 15-LOX-1 and 15-LOX-2, which catalyze the regio-selective addition of oxygen into arachidonic acid, and epidermal LOX (E-LOX) which metabolizes 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) to a specific epoxyalcohol compound. Different isozymes have different physiological roles; 5-, 12R-, 12S- and 15-LOX-1 have proliferative, antiapoptotic and angiogenic functions whilst 15-LOX-2 has roles in differentiation, growth arrest and the induction of apoptosis. E-LOX, the most divergent member of this family, is involved in terminal differentiation of keratinocytes and has a major role in skin barrier function.
Cross-Reactivity
Human
Immunogen
ALOX15 antibody was raised in Mouse using full length human recombinant protein of human ALOX15 produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALOX15 protein (Right) or empty vector (Left) detected with ALOX15 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALOX15 protein (Right) or empty vector (Left) detected with ALOX15 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of ALOX15 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using ALOX15 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of ALOX15 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using ALOX15 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ALOX15 protein using ALOX15 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ALOX15 protein using ALOX15 antibody)
Related Product Information for anti-ALOX15 antibody
Mouse monoclonal ALOX15 antibody
Product Categories/Family for anti-ALOX15 antibody

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Product Notes

The ALOX15 (Catalog #AAA838702) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ALOX15 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 IHC: 1:150 WB: 1:500-2000. Researchers should empirically determine the suitability of the ALOX15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALOX15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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