Immunohistochemistry (IHC)
(Human Skeletal Muscle, Interstitial Cells: Formalin-Fixed, Paraffin-Embedded (FFPE))
Goat anti-Human SFRP4 Polyclonal Antibody | anti-SFRP4 antibody
SFRP4, ID (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 0.3-1ug/ml
ELISA: 1:16,000
Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
Immunohistochemistry (IHC)
(Human Skeletal Muscle, Interstitial Cells: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Skeletal Muscle, Interstitial Cells: Formalin-Fixed, Paraffin-Embedded (FFPE))
Chemiluminescence
(SFRP4 antibody (0.3ug/ml) staining of human uterus lysate (35ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
(SFRP4 antibody (0.3ug/ml) staining of human uterus lysate (35ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling
By similarity. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Ref.2
Subcellular location: Secreted. Note: Cytoplasmic in ovarian tumor cells. Ref.10
Tissue specificity: Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level). Ref.9 Ref.10
Induction: Increased levels in failing myocardium. Up-regulated in several tumor types including ostomalacia-associated tumors and endometrial and breast carcinomas.
Domain: The FZ domain is involved in binding with Wnt ligands
By similarity.
Sequence similarities: Belongs to the secreted frizzled-related protein (sFRP) family.Contains 1 FZ (frizzled) domain.Contains 1 NTR domain.
Sequence caution: The sequence BAG63683.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on SFRP4
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Product Notes
The SFRP4 sfrp4 (Catalog #AAA648857) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The SFRP4, ID (Secreted Frizzled Related Protein 4, DDC-4, FrpAP, frpHE, FrzB-2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SFRP4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Western Blot: 0.3-1ug/ml ELISA: 1:16,000 Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml. Researchers should empirically determine the suitability of the SFRP4 sfrp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFRP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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