Rabbit anti-Human MOS Polyclonal Antibody | anti-MOS antibody

MOS, NT (Proto-oncogene Serine/threonine-protein Kinase Mos, MGC119962, MGC119963, MSV, Oocyte Maturation Factor Mos, Proto-oncogene c-Mos) (Azide free) (HRP)

Cat. Num. AAA6488526

• Gene Names: MOS; MSV
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MOS; Polyclonal Antibody; NT (Proto-oncogene Serine/threonine-protein Kinase Mos; MGC119962; MGC119963; MSV; Oocyte Maturation Factor Mos; Proto-oncogene c-Mos) (Azide free) (HRP); v-mos Moloney Murine Sarcoma Viral Oncogene Homolog; EC=2.7.11.1; anti-MOS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MOS.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MOS antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human MOS (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-MOS-R8 Pab in 293 and Hela cell line lysate (35ug/lane). MOS-R8(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of MOS (arrow) using rabbit polyclonal hMOS-R8. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MOS gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MOS antibody

Antibodies; Oncoproteins

References

1. Caubet, J.F., et al., EMBO J. 4(9):2245-2248 (1985). 2. Watson, R., et al., Proc. Natl. Acad. Sci. USA 79(13):4078-4082 (1982). 3. Singh, B., et al., Prog Cell Cycle Res 3, 251-259 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4885489
• NCBI GeneID: 4342
• NCBI Accession #: NP_005363
• NCBI GenBank Nucleotide #: NM_005372
• UniProt Accession #: P00540
• Molecular Weight: 38kDa
• NCBI Official Full Name: proto-oncogene serine/threonine-protein kinase mos
• NCBI Official Synonym Full Names: MOS proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: MOS
• NCBI Official Synonym Symbols: MSV
• NCBI Protein Information: proto-oncogene serine/threonine-protein kinase mos
• UniProt Protein Name: Proto-oncogene serine/threonine-protein kinase mos
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: MOS
• UniProt Entry Name: MOS_HUMAN

NCBI Description

MOS is a serine/threonine kinase that activates the MAP kinase cascade through direct phosphorylation of the MAP kinase activator MEK (MAP2K1; MIM 176872) (Prasad et al., 2008 [PubMed 18246541]).[supplied by OMIM, Jul 2009]

Uniprot Description

MOS: a proto-oncogenic serine/threonine kinase of the MOS family. Expressed predominantly in germ cells. Crucial for normal oocyte meiosis and female fertility in mice.

Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Oncoprotein; Protein kinase, Ser/Thr (non-receptor); Other group; MOS family

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; MAP kinase kinase kinase activity; ATP binding

Biological Process: establishment of meiotic spindle orientation; establishment and/or maintenance of chromatin architecture; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; regulation of meiosis; MAPKKK cascade; meiotic spindle organization and biogenesis