Rabbit anti-Human MAP3K7 Polyclonal Antibody | anti-MAP3K7 antibody

MAP3K7, CT (Mitogen-activated Protein Kinase Kinase Kinase 7, MEKK7, Transforming Growth Factor-beta-activated Kinase 1, TGF-beta-activated Kinase 1, TAK1, TGF1a) (PE)

Cat. Num. AAA6487666

• Gene Names: MAP3K7; TAK1; MEKK7; TGF1a
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAP3K7; Polyclonal Antibody; CT (Mitogen-activated Protein Kinase Kinase Kinase 7; MEKK7; Transforming Growth Factor-beta-activated Kinase 1; TGF-beta-activated Kinase 1; TAK1; TGF1a) (PE); EC=2.7.11.25; anti-MAP3K7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAK1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MAP3K7 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human TAK1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-TAK1 Pab in HL60 cell lysate. TAK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MAP3K7 antibody

Antibodies; Protein Kinases

References

1. Cheung, P.C., et al., EMBO J. 22(21):5793-5805 (2003). 2. Sakurai, H., et al., J. Biol. Chem. 278(38):36916-36923 (2003). 3. Ono, K., et al., Biochem. Biophys. Res. Commun. 307(2):332-337 (2003). 4. Edlund, S., et al., Mol. Biol. Cell 14(2):529-544 (2003). 5. Takaesu, G., et al., J. Mol. Biol. 326(1):105-115 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4507361
• NCBI GeneID: 6885
• NCBI Accession #: NP_003179.1
• NCBI GenBank Nucleotide #: NM_003188.3
• UniProt Accession #: O43318; O43317; O43319; Q5TDN2; Q5TDN3; Q5TDT7; Q9NTR3; Q9NZ70; B2RE27; E1P523
• Molecular Weight: 67,196 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 7 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 7
• NCBI Official Symbol: MAP3K7
• NCBI Official Synonym Symbols: TAK1; MEKK7; TGF1a
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 7; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; transforming growth factor-beta-activated kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 7
• Protein Family: MAP3K7 C-terminal-like protein
• UniProt Gene Name: MAP3K7
• UniProt Synonym Gene Names: TAK1; TGF-beta-activated kinase 1
• UniProt Entry Name: M3K7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TAK1: a protein kinase of the MLK family. Mediates signal transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. Also activates MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced isoforms have been reported. Three splice variant isoforms have been described.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; TAK1 subfamily

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; endosome membrane; IkappaB kinase complex; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of JNK activity; I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; positive regulation of T cell cytokine production; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; transforming growth factor beta receptor signaling pathway; JNK cascade; angiogenesis; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-2 production; toll-like receptor signaling pathway; innate immune response; positive regulation of T cell activation; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; neural tube formation