Western Blot (WB)
(THOC1 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the THOC1 antibody detected the THOC1 protein (arrow).)
Mouse anti-Human THOC1 Monoclonal Antibody | anti-THOC1 antibody
THOC1, NT (THOC1, HPR1, THO complex subunit 1, Nuclear matrix protein p84, hTREX84)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1000
Western Blot: 1:100~250
Western Blot (WB)
(THOC1 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the THOC1 antibody detected the THOC1 protein (arrow).)
Western Blot (WB)
(THOC1 Antibody (N-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the THOC1 antibody detected the THOC1 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).[supplied by OMIM, Nov 2010]
Uniprot Description
Function: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. Ref.7 Ref.8 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17Regulates transcriptional elongation of a subset of genes. Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1. Ref.7 Ref.8 Ref.9 Ref.10 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17
Subunit structure: Component of the THO complex, which is composed of THOC1, THOC2, THOC5, THOC6 and THOC7. Together with THOC3, ALYREF/THOC4 and DDX39B, THO forms the transcription/export (TREX) complex. Binds to the hypophosphorylated form of RB1. Interacts with THOC2, DDX39B and RNA polymerase II. Ref.1 Ref.10 Ref.12 Ref.13 Ref.14
Subcellular location: Isoform 1: Nucleus speckle. Nucleus › nucleoplasm. Nucleus matrix. Cytoplasm Ref.1 Ref.2 Ref.11. Note: Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution. Ref.1 Ref.2 Ref.11Isoform 2: Cytoplasm Ref.1 Ref.2 Ref.11.
Tissue specificity: Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correlates with tumor size and the metastatic state of the tumor progression. Ref.2 Ref.12
Induction: Up-regulated during cell proliferation. Ref.2
Domain: An intact death domain is needed for apoptosis. Ref.7
Post-translational modification: Expression is altered specifically during apoptosis and is accompanied by the appearance of novel forms with smaller apparent molecular mass.
Sequence similarities: Contains 1 death domain.
Research Articles on THOC1
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Product Notes
The THOC1 thoc1 (Catalog #AAA648713) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The THOC1, NT (THOC1, HPR1, THO complex subunit 1, Nuclear matrix protein p84, hTREX84) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's THOC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA. Dilution: ELISA: 1:1000 Western Blot: 1:100~250. Researchers should empirically determine the suitability of the THOC1 thoc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "THOC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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