Rabbit anti-Human Mastermind-Like 3 Polyclonal Antibody | anti-MAML3 antibody

Mastermind-Like 3, CT (MAML3, CAGH3, ERDA3, GDN, KIAA1816, MAM3, Mam-3, MGC59565, TNRC3) (Biotin)

Cat. Num. AAA6486916

• Gene Names: MAML3; GDN; MAM2; CAGH3; ERDA3; MAM-2; TNRC3; mam-3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Mastermind-Like 3; Polyclonal Antibody; CT (MAML3; CAGH3; ERDA3; GDN; KIAA1816; MAM3; Mam-3; MGC59565; TNRC3) (Biotin); Mastermind-like 3 (Drosophila); MAM2; MAM-2; anti-MAML3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAML3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MAML3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MAML3 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAML3 antibody (C-term) in Hela cell line lysates (35ug/lane). MAML3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(MAML3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymphnode followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAML3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MAML3 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MAML3 antibody

Antibodies; Transcription Factors

References

1. Tonon, G., Nat. Genet. 33(2), 208-213 (2003). 2. Lin, S.E., J. Biol. Chem. 277(52), 50612-50620 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 1393643650
• NCBI GeneID: 55534
• NCBI Accession #: NP_061187
• NCBI GenBank Nucleotide #: NM_018717
• UniProt Accession #: Q96JK9
• Molecular Weight: 122kDa
• NCBI Official Full Name: mastermind-like protein 3
• NCBI Official Synonym Full Names: mastermind like transcriptional coactivator 3
• NCBI Official Symbol: MAML3
• NCBI Official Synonym Symbols: GDN; MAM2; CAGH3; ERDA3; MAM-2; TNRC3; mam-3
• NCBI Protein Information: mastermind-like protein 3
• UniProt Protein Name: Mastermind-like protein 3
• Protein Family: Mastermind-like protein
• UniProt Gene Name: MAML3
• UniProt Synonym Gene Names: Mam-3
• UniProt Entry Name: MAML3_HUMAN

Uniprot Description

MAML3: Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Belongs to the mastermind family.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: nucleoplasm; nuclear speck; nucleus

Molecular Function: transcription coactivator activity

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression