Rabbit anti-Human MAPKAPK5 Polyclonal Antibody | anti-MAPKAPK5 antibody

MAPKAPK5 (Thr182) (Mitogen-activated Protein Kinase-activated Protein Kinase 5, MAP Kinase-activated Protein Kinase 5, MAPK-activated Protein Kinase 5, MAPKAP Kinase 5, MAPKAPK-5, p38-regulated/activated Protein Kinase, PRAK) (Biotin)

Cat. Num. AAA6486673

• Gene Names: MAPKAPK5; MK5; MK-5; PRAK; MAPKAP-K5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MAPKAPK5; Polyclonal Antibody; MAPKAPK5 (Thr182) (Mitogen-activated Protein Kinase-activated Protein Kinase 5; MAP Kinase-activated Protein Kinase 5; MAPK-activated Protein Kinase 5; MAPKAP Kinase 5; MAPKAPK-5; p38-regulated/activated Protein Kinase; PRAK) (Biotin); EC=2.7.11.1; anti-MAPKAPK5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAPKAPK5.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MAPKAPK5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding T182 of human MAPKAPK5 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAPKAPK5 Antibody (T182) in Hela cell line lysates (35ug/lane). MAPKAPK5 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of MAPKAPK5 (arrow) using rabbit polyclonal MAPKAPK5 Antibody (T182). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPKAPK5 gene.)

Product Categories/Family for anti-MAPKAPK5 antibody

Antibodies; Protein Kinases

References

1. New, L., et al., EMBO J. 17(12):3372-3384 (1998). 2. Ni, H., et al., Biochem. Biophys. Res. Commun. 243(2):492-496 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 21237765
• NCBI GeneID: 8550
• NCBI Accession #: NP_003659
• NCBI GenBank Nucleotide #: NM_003668
• UniProt Accession #: Q8IW41; O60491; Q86X46; Q9BVX9; Q9UG86; B3KVA5
• Molecular Weight: 54,035 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 5 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 5
• NCBI Official Symbol: MAPKAPK5
• NCBI Official Synonym Symbols: MK5; MK-5; PRAK; MAPKAP-K5
• NCBI Protein Information: MAP kinase-activated protein kinase 5; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAPK-5; p38-regulated/activated protein kinase
• UniProt Protein Name: MAP kinase-activated protein kinase 5
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: MAPKAPK5
• UniProt Synonym Gene Names: PRAK; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; PRAK
• UniProt Entry Name: MAPK5_HUMAN

Uniprot Description

MAPKAPK5: a member of the MAPKAPK family of protein kinases. Activated through phosphorylation by MAP kinases including ERK, p38-alpha, and MAPp38-beta In response to cellular stress and proinflammatory cytokines. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Tumor suppressor; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; p53 binding; mitogen-activated protein kinase binding; ATP binding

Biological Process: regulation of translation; negative regulation of TOR signaling pathway; activation of MAPK activity; protein amino acid autophosphorylation; Ras protein signal transduction; MAPKKK cascade; signal transduction