Rabbit anti-Human MAPKAP Kinase 2 Polyclonal Antibody | anti-MAPKAPK2 antibody

MAPKAP Kinase 2, Phosphorylated (Ser272) (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2) (MaxLight 650)

Cat. Num. AAA6486657

• Gene Names: MAPKAPK2; MK2; MK-2; MAPKAP-K2
• Reactivity: Human
• Applications: Dot Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: MAPKAP Kinase 2; Polyclonal Antibody; Phosphorylated (Ser272) (Mitogen-activated Protein Kinase-activated Protein Kinase 2; MAP Kinase-activated Protein Kinase 2; MAPK-activated Protein Kinase 2; MAPKAPK2; MAPKAPK-2; MK2) (MaxLight 650); EC=2.7.11.1; anti-MAPKAPK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAPKAPK2 when phosphorylated at Ser272.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-MAPKAPK2 antibody: FLISA, Dot Blot (DB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues surrounding Ser272 of human MAPKAPK2. UniProt Accession #P49137. Species sequence homology: mouse and rabbit
• Conjugate: MaxLight650
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis on nitrocellulose membrane using M2355-01 (0.5ug/ml). Top: Non Phospho-peptide (50ng) Bottom: Phospho-peptide (50ng) )

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human cancer tissue using MBS626761 followed by peroxidase conjugated of the secondary antibody and AEC staining. This data demonstrates the use of MBS626761 for immunohistochemistry.)

Related Product Information for anti-MAPKAPK2 antibody

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-MAPKAPK2 antibody

Antibodies; Protein Kinases

References

1. Lukas, S.M., et al., Biochemistry 43(31):9950-9960 (2004). 2. Underwood, K.W., et al., Structure (Camb.) 11(6):627-636 (2003). 3. Meng, W., et al., J. Biol. Chem. 277(40):37401-37405 (2002). 4. Han, Q., et al., J. Biol. Chem. 277(50):48379-48385 (2002). 5. Werz, O., et al., Proc. Natl. Acad. Sci. USA 97(10):5261-5266 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 32481209
• NCBI GeneID: 9261
• NCBI Accession #: NP_116584
• NCBI GenBank Nucleotide #: NM_032960.3
• UniProt Accession #: P49137; Q5SY30; Q5SY41; Q8IYD6
• Molecular Weight: 42,203 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 2 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 2
• NCBI Official Symbol: MAPKAPK2
• NCBI Official Synonym Symbols: MK2; MK-2; MAPKAP-K2
• NCBI Protein Information: MAP kinase-activated protein kinase 2
• UniProt Protein Name: MAP kinase-activated protein kinase 2
• UniProt Gene Name: MAPKAPK2
• UniProt Synonym Gene Names: MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2
• UniProt Entry Name: MAPK2_HUMAN

NCBI Description

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MAPKAPK2: a member of the MAPKAPK family of protein kinases. It is phosphorylated and activated by p38 in response to cytokines, stress and chemotactic factors.MAPKAPK-2 is apparently a direct target of p38 MAPK. HSP27 is one of its in vivo substrates. Two transcript variants encoding two different isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; signal transducer activity; protein binding; ATP binding; protein kinase activity

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; stress-activated MAPK cascade; leukotriene metabolic process; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of interleukin-6 production; arachidonic acid metabolic process; response to stress; inflammatory response; inner ear development; toll-like receptor 4 signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cytokine stimulus; Ras protein signal transduction; toll-like receptor signaling pathway; innate immune response; gene expression; regulation of tumor necrosis factor production; toll-like receptor 9 signaling pathway; G2/M transition DNA damage checkpoint; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus