Rabbit anti-Human MAP2K4 Polyclonal Antibody | anti-MAP2K4 antibody

MAP2K4 (Ser257) (Dual Specificity Mitogen-activated Protein Kinase Kinase 4, MAP Kinase Kinase 4, MAPKK 4, MAPKK4, MKK4, JNK-activating Kinase 1, c-Jun N-terminal Kinase Kinase 1, JNKK1, JNKK, MAPK/ERK Kinase 4, MEK 4, MEK4, PRKMK4, SAPK/ERK Kinase 1, SEK

Cat. Num. AAA6486494

• Gene Names: MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: MAP2K4; Polyclonal Antibody; MAP2K4 (Ser257) (Dual Specificity Mitogen-activated Protein Kinase Kinase 4; MAP Kinase Kinase 4; MAPKK 4; MAPKK4; MKK4; JNK-activating Kinase 1; c-Jun N-terminal Kinase Kinase 1; JNKK1; JNKK; MAPK/ERK Kinase 4; MEK 4; MEK4; PRKMK4; SAPK/ERK Kinase 1; SEK; EC=2.7.12.2; Mitogen-activated Protein Kinase Kinase 4; anti-MAP2K4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP2K4.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MAP2K4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic non-phosphopeptide corresponding to amino acid residues surrounding Ser257of human MAP2K4 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MAP2K4 (arrow) using rabbit polyclonal MAP2K4 Antibody (S257).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-MAP2K4 antibody

Antibodies; Protein Kinases

References

1. Robinson, V.L., Mol. Cancer Res. 6(3), 501-508 (2008). 2. Zhang, H., J. Biol. Chem. 282(20), 14788-14796 (2007). 3. Salmeron, A., EMBO J. 15(4), 817-826 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4506889
• NCBI GeneID: 6416
• NCBI Accession #: NP_003001
• NCBI GenBank Nucleotide #: NM_003010
• UniProt Accession #: P45985
• Molecular Weight: 44kDa
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 4 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: MAP2K4
• NCBI Official Synonym Symbols: JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K4
• UniProt Synonym Gene Names: JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; MAP kinase kinase 4; MAPKK 4; MEK 4; SEK1; SAPK kinase 1; SAPKK-1; SAPKK1; JNKK
• UniProt Entry Name: MP2K4_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.

Protein type: Kinase, protein; EC 2.7.12.2; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 17p12

Cellular Component: dendrite cytoplasm; axon; perikaryon; intracellular; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; activation of JNK activity; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of DNA replication; positive regulation of nitric-oxide synthase biosynthetic process