Rabbit anti-Human Luteinizing Hormone, beta Polyclonal Antibody | anti-LHB antibody

Luteinizing Hormone, beta, CT (Luteinizing Hormone Subunit beta, LHB, LH-B, hLHB, LSH-beta, LSH-B, CGB4, Lutropin beta Chain, Lutropin Subunit beta) (MaxLight 490)

Cat. Num. AAA6485578

• Gene Names: LHB; CGB4; HH23; hLHB; LSH-B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Luteinizing Hormone; beta; Polyclonal Antibody; CT (Luteinizing Hormone Subunit beta; LHB; LH-B; hLHB; LSH-beta; LSH-B; CGB4; Lutropin beta Chain; Lutropin Subunit beta) (MaxLight 490); Luteinizing Hormone beta Polypeptide; Luteinising Hormone; anti-LHB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Luteinizing Hormone, beta.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-LHB antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA
• Application Notes: IHC-P: 1:10-1:50; WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 78-104 in the C-terminal region of human Luteinizing Hormone, beta. UniProt Accession # P01229.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS624721. This demonstrates that MBS624721 detected the LHB protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue usingMBS624721, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS624721 for immunohistochemistry.)

Product Categories/Family for anti-LHB antibody

Antibodies; Hormones; Steroids

References

1. Lofrano-Porto, A., N. Engl. J. Med. 357(9), 897-904 (2007). 2. Liu, S., Endocr. J. 52(6), 781-784 (2005). 3. Keutmann, H.T., Mol. Endocrinol. 6(6), 904-913 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4504989
• NCBI GeneID: 3972
• NCBI Accession #: NP_000885
• NCBI GenBank Nucleotide #: NM_000894.2
• UniProt Accession #: P01229; Q9UDI0
• Molecular Weight: 15,345 Da
• NCBI Official Full Name: lutropin subunit beta
• NCBI Official Synonym Full Names: luteinizing hormone beta polypeptide
• NCBI Official Symbol: LHB
• NCBI Official Synonym Symbols: CGB4; HH23; hLHB; LSH-B
• NCBI Protein Information: lutropin subunit beta
• UniProt Protein Name: Lutropin subunit beta
• Protein Family: Lutropin/choriogonadotropin
• UniProt Gene Name: LHB
• UniProt Synonym Gene Names: Lutropin beta chain; LH-B; LSH-B; LSH-beta
• UniProt Entry Name: LSHB_HUMAN

NCBI Description

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism. [provided by RefSeq, Jul 2008]

Uniprot Description

LHB: Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. Defects in LHB are a cause of hypogonadism LHB-related (HGON-LHB). Hypogonadism is characterized by infertility and pseudohermaphroditism. Belongs to the glycoprotein hormones subunit beta family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: progesterone biosynthetic process; cellular protein metabolic process; cell-cell signaling; male gonad development; peptide hormone processing; signal transduction

Disease: Fertile Eunuch Syndrome