Rabbit anti-Human LTA4H Polyclonal Antibody | anti-LTA4H antibody

LTA4H, ID (Leukotriene A-4 Hydrolase, Leukotriene A(4) Hydrolase, LTA-4 Hydrolase, LTA4) (MaxLight 750)

Cat. Num. AAA6485024

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: LTA4H; Polyclonal Antibody; ID (Leukotriene A-4 Hydrolase; Leukotriene A(4) Hydrolase; LTA-4 Hydrolase; LTA4) (MaxLight 750); anti-LTA4H antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LTA4H.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-LTA4H antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human LTA4H (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of LTA4H Antibody (Center) in T47D cell line lysates (35ug/lane). LTA4H (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lymph tissue reacted with CORO1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of LTA4H Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-LTA4H antibody

Antibodies; Enzymes

References

1. Bevan, S., Stroke 40(3), 696-701 (2009). 2. Crosslin, D.R., Hum. Genet. 125(2), 217-229 (2009). 3. Huston, A.L., Biochim. Biophys. Acta 1784(11), 1865-1872 (2008). 4. Rybina, I.V., J. Biol. Chem. 272(50), 31865-31871 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 4505029
• NCBI GeneID: 4048
• NCBI Accession #: NP_000886
• NCBI GenBank Nucleotide #: NM_000895.2
• UniProt Accession #: P09960; Q6IAT6; Q9UCT7; B4DNQ9; F8VV40
• Molecular Weight: 71.7kDa (634aa)
• NCBI Official Full Name: leukotriene A-4 hydrolase isoform 1
• NCBI Official Synonym Full Names: leukotriene A4 hydrolase
• NCBI Official Symbol: LTA4H
• NCBI Protein Information: leukotriene A-4 hydrolase
• UniProt Protein Name: Leukotriene A-4 hydrolase
• Protein Family: Leukotriene A-4 hydrolase
• UniProt Gene Name: LTA4H
• UniProt Synonym Gene Names: LTA4; LTA-4 hydrolase
• UniProt Entry Name: LKHA4_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.3.2.6; Hydrolase; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: peptidase activity; leukotriene-A4 hydrolase activity; metallopeptidase activity; zinc ion binding; epoxide hydrolase activity; aminopeptidase activity

Biological Process: leukotriene biosynthetic process; peptide catabolic process; arachidonic acid metabolic process; leukotriene metabolic process; proteolysis; inflammatory response