Rabbit anti-Human Lamin B2 Polyclonal Antibody | anti-LMNB2 antibody

Lamin B2, CT (Lamin-B2, LAMB2, LMN2, LMNB2, MGC2721) (PE)

Cat. Num. AAA6484667

• Gene Names: LMNB2; LMN2; LAMB2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Lamin B2; Polyclonal Antibody; CT (Lamin-B2; LAMB2; LMN2; LMNB2; MGC2721) (PE); anti-LMNB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Lamin B2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-LMNB2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human Lamin B2 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Lamin B2 Antibody (C-term) in Y79 cell line lysates (35ug/lane). Lamin B2 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-LMNB2 antibody

Antibodies; Lamin; Laminin

References

1. Schumacher, J., FEBS Lett. 580(26), 6211-6216 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 388240801
• NCBI GeneID: 84823
• NCBI Accession #: NP_116126
• NCBI GenBank Nucleotide #: NM_032737
• UniProt Accession #: Q03252; O75292; Q14734; Q96DF6
• Molecular Weight: 67,689 Da
• NCBI Official Full Name: lamin-B2
• NCBI Official Synonym Full Names: lamin B2
• NCBI Official Symbol: LMNB2
• NCBI Official Synonym Symbols: LMN2; LAMB2
• NCBI Protein Information: lamin-B2; lamin B3
• UniProt Protein Name: Lamin-B2
• Protein Family: Lamin
• UniProt Gene Name: LMNB2
• UniProt Synonym Gene Names: LMN2
• UniProt Entry Name: LMNB2_HUMAN

Uniprot Description

Lamin B2: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Lamin B2 may be lost during the follicle centre stage of B-cell differentiation in humans. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: lamin filament; nuclear inner membrane

Molecular Function: structural molecule activity

Disease: Lipodystrophy, Partial, Acquired, Susceptibility To