Rabbit anti-Human, Mouse KLF5 Polyclonal Antibody | anti-KLF5 antibody

KLF5, CT (Kruppel-like Factor 5, Basic Transcription Element Binding Protein 2, BTE-binding Protein 2, BTEB2, Colon Krueppel-like Factor, CKLF, GC Box Binding Protein 2, Intestinal-enriched Krueppel-like Factor, IKLF, Transcription Factor BTEB2) (Biotin)

Cat. Num. AAA6484450

• Gene Names: KLF5; CKLF; IKLF; BTEB2
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: KLF5; Polyclonal Antibody; CT (Kruppel-like Factor 5; Basic Transcription Element Binding Protein 2; BTE-binding Protein 2; BTEB2; Colon Krueppel-like Factor; CKLF; GC Box Binding Protein 2; Intestinal-enriched Krueppel-like Factor; IKLF; Transcription Factor BTEB2) (Biotin); Kruppel-like Factor 5 (Intestinal); anti-KLF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KLF5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-KLF5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human KLF5 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KLF5 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLF5 antibody detected the KLF5 protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of U251 cell stained with KLF5 Antibody (C-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with KLF5 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.)

Immunohistochemistry (IHC)

(KLF5 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLF5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KLF5 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KLF5 antibody

Antibodies; Transcription Factors

References

1. Guo, P., Dong, X.Y. J. Biol. Chem. 284(10), 6071-6078 (2009). 2. Lee, M.Y., Moon, J.S. Biochem. J. 417(1), 313-322 (2009). 3. Du, J.X., Bialkowska, A.B. J. Biol. Chem. 283(46), 31991-32002 (2008). 4. Miyamoto, S., Suzuki, T. Mol. Cell. Biol. 23(23), 8528-8541 (2003). 5. Shi, H., Zhang, Z. Nucleic Acids Res. 27(24), 4807-4815 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 14251215
• NCBI GeneID: 688
• NCBI Accession #: NP_001721
• NCBI GenBank Nucleotide #: NM_001730
• UniProt Accession #: Q13887; Q9UHP8; L0R3U5; L0R4T9
• Molecular Weight: 18,116 Da
• NCBI Official Full Name: Krueppel-like factor 5 isoform 1
• NCBI Official Synonym Full Names: Kruppel-like factor 5 (intestinal)
• NCBI Official Symbol: KLF5
• NCBI Official Synonym Symbols: CKLF; IKLF; BTEB2
• NCBI Protein Information: Krueppel-like factor 5; BTE-binding protein 2; GC box binding protein 2; Klf5C isoform; basic transcription element binding protein 2; colon krueppel-like factor; colon kruppel-like factor; intestinal-enriched krueppel-like factor; intestinal-enriched kru
• UniProt Protein Name: Krueppel-like factor 5
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF5
• UniProt Synonym Gene Names: BTEB2; CKLF; IKLF; BTE-binding protein 2
• UniProt Entry Name: KLF5_HUMAN

Uniprot Description

KLF5: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q22.1

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm; plasma membrane

Molecular Function: protein binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; microvillus biogenesis; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; regulation of microvillus biogenesis