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Western Blot (WB) (Western blot analysis of IL8 Antibody (C-term) in MDA-MB468 cell line lysates (35ug/lane). IL8 (arrow) was detected using the purified Pab.)

Rabbit anti-Human IL8 Polyclonal Antibody | anti-IL8 antibody

IL8, CT (Interleukin-8, IL-8, C-X-C Motif Chemokine 8, Monocyte-derived Neutrophil Chemotactic Factor, MDNCF, T-cell Chemotactic Factor, Neutrophil-activating Protein 1, NAP-1, Protein 3-10C, Granulocyte Chemotactic Protein 1, GCP-1, Monocyte-derived Neut

Gene Names
CXCL8; IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
IL8; Polyclonal Antibody; CT (Interleukin-8; IL-8; C-X-C Motif Chemokine 8; Monocyte-derived Neutrophil Chemotactic Factor; MDNCF; T-cell Chemotactic Factor; Neutrophil-activating Protein 1; NAP-1; Protein 3-10C; Granulocyte Chemotactic Protein 1; GCP-1; Monocyte-derived Neut; anti-IL8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IL8.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-IL8 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human IL8 (KLH).
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of IL8 Antibody (C-term) in MDA-MB468 cell line lysates (35ug/lane). IL8 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of IL8 Antibody (C-term) in MDA-MB468 cell line lysates (35ug/lane). IL8 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IL8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IL8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of IL8 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of IL8 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(IL8 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (IL8 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-IL8 antibody
References
1. Baggiolini, M.et.al., FEBS Lett. 307(1), 97-101 (1992). 2. Holmes, W.E., et.al., Science 253(5025), 1278-1280 (1991).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9kDa (78aa), confirmed by MALDI-TOF.
NCBI Official Full Name
interleukin-8 isoform 1
NCBI Official Synonym Full Names
C-X-C motif chemokine ligand 8
NCBI Official Symbol
CXCL8
NCBI Official Synonym Symbols
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
NCBI Protein Information
interleukin-8
UniProt Protein Name
Interleukin-8
UniProt Gene Name
IL8
UniProt Synonym Gene Names
CXCL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF
UniProt Entry Name
IL8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is secreted primarily by neutrophils, where it serves as a chemotactic factor by guiding the neutrophils to the site of infection. This chemokine is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, Aug 2017]

Research Articles on IL8

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Product Notes

The IL8 il8 (Catalog #AAA6482844) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL8, CT (Interleukin-8, IL-8, C-X-C Motif Chemokine 8, Monocyte-derived Neutrophil Chemotactic Factor, MDNCF, T-cell Chemotactic Factor, Neutrophil-activating Protein 1, NAP-1, Protein 3-10C, Granulocyte Chemotactic Protein 1, GCP-1, Monocyte-derived Neut reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IL8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the IL8 il8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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