Rabbit anti-Human MAGEA3 Polyclonal Antibody | anti-MAGEA3 antibody

MAGEA3, CT (MAGEA3, MAGE3, Melanoma-associated antigen 3, Antigen MZ2-D, Cancer/testis antigen 1.3, MAGE-3 antigen)

Cat. Num. AAA648213

• Gene Names: MAGEA3; HIP8; HYPD; CT1.3; MAGE3; MAGEA6
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: MAGEA3; Polyclonal Antibody; CT (MAGEA3; MAGE3; Melanoma-associated antigen 3; Antigen MZ2-D; Cancer/testis antigen 1.3; MAGE-3 antigen); Anti -MAGEA3; anti-MAGEA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-MAGEA3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50; Immunohistochemistry: 1:10-50
• Immunogen: MAGEA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285~314 amino acids from the C-terminal region of human MAGEA3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot to detect MAGEA3 in Jurkat lysate using MBS648213.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS648213 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Jurkat cells using MBS648213 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MAGEA3 antibody

MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.

Product Categories/Family for anti-MAGEA3 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4885467
• NCBI GeneID: 4102
• NCBI Accession #: NP_005353.1
• NCBI GenBank Nucleotide #: NM_005362.3
• UniProt Accession #: P43357; Q6FHI6
• Molecular Weight: 34,747 Da
• NCBI Official Full Name: melanoma-associated antigen 3
• NCBI Official Synonym Full Names: melanoma antigen family A, 3
• NCBI Official Symbol: MAGEA3
• NCBI Official Synonym Symbols: HIP8; HYPD; CT1.3; MAGE3; MAGEA6
• NCBI Protein Information: melanoma-associated antigen 3; antigen MZ2-D; MAGE-3 antigen; cancer/testis antigen 1.3; cancer/testis antigen family 1, member 3
• UniProt Protein Name: Melanoma-associated antigen 3
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEA3
• UniProt Synonym Gene Names: MAGE3; CT1.3
• UniProt Entry Name: MAGA3_HUMAN

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. Ref.9

Subunit structure: Interacts with TRIM28. Ref.8 Ref.9

Tissue specificity: Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.

Sequence similarities: Contains 1 MAGE domain.

Caution: In vitro experiments by Ref.8 measuring cell viability in melanoma cell lines used siRNA specific for MAGEA3 and MAGEA6.