Rabbit anti-Human ILK Polyclonal Antibody | anti-ILK antibody

ILK, CT (Integrin Linked Kinase, Integrin-linked Protein Kinase, 59kD Serine/threonine Protein Kinase, DKFZp686F1765, ILK 1, ILK1, ILK-1, ILK 2, ILK2, ILK-2, p59, p59ILK) (FITC)

Cat. Num. AAA6481574

• Gene Names: Ilk; ESTM24; AA511515
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ILK; Polyclonal Antibody; CT (Integrin Linked Kinase; Integrin-linked Protein Kinase; 59kD Serine/threonine Protein Kinase; DKFZp686F1765; ILK 1; ILK1; ILK-1; ILK 2; ILK2; ILK-2; p59; p59ILK) (FITC); anti-ILK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ILK1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ILK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ILK1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-ILK antibody

Antibodies; Protein Kinases

References

1. Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003). 2. Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003). 3. Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003). 4. Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003). 5. Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 118595638
• NCBI GeneID: 16202
• UniProt Accession #: O55222; Q78KK2
• Molecular Weight: 51,373 Da
• NCBI Official Full Name: Integrin-linked protein kinase
• NCBI Official Synonym Full Names: integrin linked kinase
• NCBI Official Symbol: Ilk
• NCBI Official Synonym Symbols: ESTM24; AA511515
• NCBI Protein Information: integrin-linked protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• Protein Family: Integrin-linked protein kinase
• UniProt Gene Name: Ilk
• UniProt Entry Name: ILK_MOUSE

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: EC 2.7.11.1; Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; ILK subfamily

Cellular Component: axon; cell junction; cell projection; cell soma; costamere; cytoplasm; dendritic shaft; extracellular matrix; focal adhesion; intercellular junction; lamellipodium; membrane; nucleoplasm; plasma membrane; protein complex; stress fiber; terminal button

Molecular Function: ATP binding; integrin binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; SH3 domain binding; signal transducer activity; transferase activity

Biological Process: cell aging; cell cycle arrest; cell projection organization and biogenesis; establishment and/or maintenance of epithelial cell polarity; fibril organization and biogenesis; integrin-mediated signaling pathway; myelin formation; myelination in the peripheral nervous system; negative regulation of apoptosis; negative regulation of neuron apoptosis; negative regulation of protein kinase activity; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; nerve development; neurite morphogenesis; peptidyl-serine phosphorylation; phosphorylation; positive regulation of axon extension; positive regulation of axonogenesis; positive regulation of BMP signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of dendrite morphogenesis; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of myoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein heterooligomerization; protein kinase B signaling cascade; regulation of actin cytoskeleton organization and biogenesis; regulation of cell growth; Schwann cell development; ureteric bud branching