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Western Blot (WB) (Western blot analysis of GNPAT Antibody (C-term) in 293 cell line lysates (35ug/lane). GNPAT (arrow) was detected using the purified Pab.)

Rabbit anti-Human GNPAT Polyclonal Antibody | anti-GNPAT antibody

GNPAT, CT (Glycerone-phosphate O-acyltransferase, Acyl-CoA:dihydroxyacetonephosphateacyltransferase, Dihydroxyacetone Phosphate Acyltransferase, DAPAT, DAP-AT, DHAPAT, DHAP-AT) (APC)

Gene Names
GNPAT; DAPAT; DAP-AT; DHAPAT
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography
Synonyms
GNPAT; Polyclonal Antibody; CT (Glycerone-phosphate O-acyltransferase; Acyl-CoA:dihydroxyacetonephosphateacyltransferase; Dihydroxyacetone Phosphate Acyltransferase; DAPAT; DAP-AT; DHAPAT; DHAP-AT) (APC); Glyceronephosphate O-acyltransferase; EC=2.3.1.42; anti-GNPAT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human GNPAT.
Purity/Purification
Purified by Protein A Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-GNPAT antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
FLISA: 1:1000
IHC: 1:50-1:100
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide corresponding to a portion within aa651-680 from the C-terminal region of human GNPAT conjugated to KLH.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GNPAT Antibody (C-term) in 293 cell line lysates (35ug/lane). GNPAT (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of GNPAT Antibody (C-term) in 293 cell line lysates (35ug/lane). GNPAT (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with GNPAT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with GNPAT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GNPAT Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (GNPAT Antibody (C-term) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-GNPAT antibody
References
1. Ofman, R., et.al., Biochem. Biophys. Res. Commun. 281(3), 754-760 (2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
77,188 Da
NCBI Official Full Name
dihydroxyacetone phosphate acyltransferase
NCBI Official Synonym Full Names
glyceronephosphate O-acyltransferase
NCBI Official Symbol
GNPAT
NCBI Official Synonym Symbols
DAPAT; DAP-AT; DHAPAT
NCBI Protein Information
dihydroxyacetone phosphate acyltransferase; DHAP-AT; glycerone-phosphate O-acyltransferase; acyl-CoA:dihydroxyacetonephosphateacyltransferase
UniProt Protein Name
Dihydroxyacetone phosphate acyltransferase
UniProt Gene Name
GNPAT
UniProt Synonym Gene Names
DAPAT; DHAPAT; DAP-AT; DHAP-AT
UniProt Entry Name
GNPAT_HUMAN

NCBI Description

This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. [provided by RefSeq, Jul 2008]

Uniprot Description

GNPAT: Defects in GNPAT are the cause of rhizomelic chondrodysplasia punctata type 2 (RCDP2). RDCP2 is characterized by rhizomelic shortening of femur and humerus, vertebral disorders, cataract, cutaneous lesions and severe mental retardation. Belongs to the GPAT/DAPAT family.

Protein type: EC 2.3.1.42; Lipid Metabolism - glycerophospholipid; Transferase

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: peroxisomal membrane; peroxisomal matrix; membrane; mitochondrion; peroxisome

Molecular Function: glycerone-phosphate O-acyltransferase activity; palmitoyl-CoA hydrolase activity; receptor binding

Biological Process: response to starvation; response to drug; synaptogenesis; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; cerebellum morphogenesis; cellular lipid metabolic process; response to nutrient; ether lipid biosynthetic process; paranodal junction assembly

Disease: Rhizomelic Chondrodysplasia Punctata, Type 2

Research Articles on GNPAT

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Product Notes

The GNPAT gnpat (Catalog #AAA6478683) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GNPAT, CT (Glycerone-phosphate O-acyltransferase, Acyl-CoA:dihydroxyacetonephosphateacyltransferase, Dihydroxyacetone Phosphate Acyltransferase, DAPAT, DAP-AT, DHAPAT, DHAP-AT) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GNPAT can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-1:50 FLISA: 1:1000 IHC: 1:50-1:100 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the GNPAT gnpat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNPAT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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