Goat anti-Human, Mouse GFAP Polyclonal Antibody | anti-GFAP antibody

GFAP (Glial Fibrillary Acidic Protein) (Biotin)

Cat. Num. AAA6477723

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: GFAP; Polyclonal Antibody; GFAP (Glial Fibrillary Acidic Protein) (Biotin); anti-GFAP antibody

• Host: Goat
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GFAP. Species Crossreactivity: mouse
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GFAP antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to C-DGEVIKESKQEHKD, from the C-terminal of human GFAPl. Species sequence homology: canine, rat
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of mouse brain lysate (35ug protein in RIPA buffer)using G2032-27Q (0.03ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)

Testing Data

(G2032-27Q (5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml). Please note this data was obtained using a previous batch.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin embedded human cerebellum using G2032-27Q (2ug/ml). Steamed antigen retrieval with citrate buffer pH6, HRP-staining.)

Related Product Information for anti-GFAP antibody

GFAP (Glial Fibrillary Acidic Protein) is a member of the intermediate filament protein family which includes desmin, peripherin and vimentin. It is a major protein component in multiple sclerosis plaques but is found to be up-regulated in other instances where there has been damage or the occurrence of disease to the nervous system. GFAP is expressed in cells of astrocytic lineage, peripheral ganglia, and non-myelinating Schwann cells. In addition, neural stem cells also express GFAP. GFAP runs on SDS-PAGE gels at ~55kD, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene. Antibodies to GFAP are useful markers of astrocytic cells and neural stem cells. In addition many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene. All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.

Product Categories/Family for anti-GFAP antibody

Antibodies; Growth Factors; Cytokines

References

1. Korolainen MA, Auriola S, Nyman TA, Alafuzoff I, Pirttila T. Proteomic analysis of glial fibrillary acidic protein in Alzheimer's disease and aging brain. Neurobiol Dis. 2005 Dec;20(3):858-70. Epub 2005 Jun 27.

NCBI and Uniprot Product Information

• NCBI GI #: 4503979
• NCBI GeneID: 2670
• NCBI Accession #: NP_002046.1
• NCBI GenBank Nucleotide #: NM_002055.4
• UniProt Accession #: P14136; Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59; E9PAX3
• NCBI Official Full Name: glial fibrillary acidic protein isoform 1
• NCBI Official Synonym Full Names: glial fibrillary acidic protein
• NCBI Official Symbol: GFAP
• NCBI Protein Information: glial fibrillary acidic protein
• UniProt Protein Name: Glial fibrillary acidic protein
• Protein Family: Glial fibrillary acidic protein
• UniProt Gene Name: GFAP
• UniProt Synonym Gene Names: GFAP
• UniProt Entry Name: GFAP_HUMAN

NCBI Description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; cytoplasm; intermediate filament; cytosol

Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding

Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development

Disease: Alexander Disease