Rabbit anti-Human FLNA Polyclonal Antibody | anti-FLNA antibody

FLNA (Y1046) (Filamin-A, FLN-A, Alpha-filamin, Filamin-1, Endothelial Actin-binding Protein, Actin-binding Protein 280, ABP-280, Non-muscle Filamin, FLN, FLN1) (FITC)

Cat. Num. AAA6476546

• Gene Names: FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: FLNA; Polyclonal Antibody; FLNA (Y1046) (Filamin-A; FLN-A; Alpha-filamin; Filamin-1; Endothelial Actin-binding Protein; Actin-binding Protein 280; ABP-280; Non-muscle Filamin; FLN; FLN1) (FITC); anti-FLNA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FLNA.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 2639

• Applicable Applications for anti-FLNA antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to amino acid residues surrounding Y1046 of human FLNA (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FLNA Antibody (Y1046) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the FLNA antibody detected the FLNA protein (arrow).)

Immunohistochemistry (IHC)

(FLNA Antibody (Y1046) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FLNA Antibody (Y1046) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FLNA Antibody (Y1046) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(FLNA Antibody (Y1046) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-FLNA antibody

Antibodies; Actin Related Proteins

References

1. Ravid, D., Exp. Cell Res. 314(15), 2762-2773 (2008). 2. Maceyka, M., Mol. Cell. Biol. 28(18), 5687-5697 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 116063573
• NCBI GeneID: 2316
• NCBI Accession #: NP_001447
• NCBI GenBank Nucleotide #: NP_001447.2
• UniProt Accession #: P21333
• Molecular Weight: 280.739
• NCBI Official Full Name: filamin-A isoform 1
• NCBI Official Synonym Full Names: filamin A
• NCBI Official Symbol: FLNA
• NCBI Official Synonym Symbols: FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FGS2; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
• NCBI Protein Information: filamin-A
• UniProt Protein Name: Filamin-A
• Protein Family: Filamin
• UniProt Gene Name: FLNA
• UniProt Synonym Gene Names: FLN; FLN1; FLN-A; ABP-280
• UniProt Entry Name: FLNA_HUMAN

NCBI Description

The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Uniprot Description

FLNA: a ubiquitous cytoskeletal protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Plays an essential role in embryonic cell migration. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interactions with filamin A may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cortical cytoskeleton; focal adhesion; extracellular region; actin filament; trans-Golgi network; cytosol; actin cytoskeleton; cell soma; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus

Molecular Function: Rho GTPase binding; protein homodimerization activity; Ral GTPase binding; Rac GTPase binding; transcription factor binding; small GTPase binding; actin filament binding; protein binding; signal transducer activity; protein kinase C binding; mu-type opioid receptor binding; glycoprotein binding; SMAD binding

Biological Process: actin crosslink formation; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; negative regulation of transcription factor activity; receptor clustering; mRNA transcription from RNA polymerase II promoter; dopamine receptor, adenylate cyclase inhibiting pathway; establishment of protein localization; platelet degranulation; early endosome to late endosome transport; actin cytoskeleton reorganization; positive regulation of transcription factor import into nucleus; cytoplasmic sequestering of protein; epithelial to mesenchymal transition; cilium biogenesis; blood coagulation; negative regulation of protein catabolic process

Disease: Fg Syndrome 2; Terminal Osseous Dysplasia; Cardiac Valvular Dysplasia, X-linked; Frontometaphyseal Dysplasia; Melnick-needles Syndrome; Heterotopia, Periventricular, Ehlers-danlos Variant; Intestinal Pseudoobstruction, Neuronal, Chronic Idiopathic, X-linked; Heterotopia, Periventricular, X-linked Dominant; Otopalatodigital Syndrome, Type Ii; Otopalatodigital Syndrome, Type I