Rabbit anti-Human FGR Polyclonal Antibody | anti-FGR antibody

FGR, NT (Proto-oncogene c-Fgr, Tyrosine-protein Kinase Fgr, P55-FGR, SRC2) (Biotin)

Cat. Num. AAA6475936

• Gene Names: FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: FGR; Polyclonal Antibody; NT (Proto-oncogene c-Fgr; Tyrosine-protein Kinase Fgr; P55-FGR; SRC2) (Biotin); anti-FGR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FGR.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-FGR antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human FGR (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-FGR Pab is used in Western blot to detect FGR in Ramos cell lysate.)

Western Blot (WB)

(Western blot analysis of FGR (arrow) using FGR Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGR gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of FGR Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-FGR antibody

Antibodies; Protein Kinases

References

1. Carriero, M.V., et al., Biol. Chem. 383(1):107-113 (2002). 2. Katamine, S., et al., Mol. Cell. Biol. 8(1):259-266 (1988). 3. Nishizawa, M., et al., Mol. Cell. Biol. 6(2):511-517 (1986). 4. Cheah, M.S., et al., Nature 319(6050):238-240 (1986). 5. Tronick, S.R., et al., Proc. Natl. Acad. Sci. USA 82(19):6595-6599 (1985). 6.Oleg Tatarov, et al. Clin. Cancer Res. published 15 May 2009, 10.1158/1078-0432. CCR-08-1857. (E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 112382241
• NCBI GeneID: 2268
• NCBI Accession #: NP_001036194.1
• NCBI GenBank Nucleotide #: NM_001042729.1
• UniProt Accession #: P09769; Q9UIQ3; D3DPL7
• Molecular Weight: 59,479 Da
• NCBI Official Full Name: tyrosine-protein kinase Fgr
• NCBI Official Synonym Full Names: Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
• NCBI Official Symbol: FGR
• NCBI Official Synonym Symbols: SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
• NCBI Protein Information: tyrosine-protein kinase Fgr; p55-c-fgr protein; c-fgr protooncogene; proto-oncogene c-Fgr; c-src-2 proto-oncogene; proto-oncogene tyrosine-protein kinase FGR
• UniProt Protein Name: Tyrosine-protein kinase Fgr
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: FGR
• UniProt Synonym Gene Names: SRC2
• UniProt Entry Name: FGR_HUMAN

NCBI Description

This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FGR: a non-receptor tyrosine kinase of the Src family. Contains one SH2 and one SH3 domain. Beta 2 integrin signaling induces its c-Cbl-mediated ubiquitination in adherent neutrophils. An ortholog of v-fgr feline oncogene. Mildly amplified in hormone-resistant prostate cancer. Copy number either increased or decreased in other cancers.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Mitochondrial; Oncoprotein; Protein kinase, tyrosine (non-receptor); TK group; Src family

Chromosomal Location of Human Ortholog: 1p36.2-p36.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; mitochondrial inner membrane; plasma membrane; mitochondrial intermembrane space; cytosol; actin cytoskeleton

Molecular Function: protein binding; phosphotyrosine binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein kinase binding; ATP binding

Biological Process: integrin-mediated signaling pathway; cell migration; peptidyl-tyrosine phosphorylation; response to virus; protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase activity; immune response-regulating cell surface receptor signaling pathway; positive regulation of mast cell degranulation; regulation of phagocytosis; protein amino acid phosphorylation; regulation of innate immune response; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; regulation of cell shape; defense response to Gram-positive bacterium; innate immune response; regulation of protein kinase activity; blood coagulation; cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; positive regulation of cytokine secretion