Rabbit anti-Human FABP4 Polyclonal Antibody | anti-FABP4 antibody

FABP4, Phosphorylated (Tyr20) (Fatty Acid-binding Protein, Adipocyte, Adipocyte-type Fatty Acid-binding Protein, A-FABP, AFABP, Fatty Acid-binding Protein 4, Adipocyte Lipid-binding Protein, ALBP) (PE)

Cat. Num. AAA6475652

• Gene Names: FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FABP4; Polyclonal Antibody; Phosphorylated (Tyr20) (Fatty Acid-binding Protein; Adipocyte; Adipocyte-type Fatty Acid-binding Protein; A-FABP; AFABP; Fatty Acid-binding Protein 4; Adipocyte Lipid-binding Protein; ALBP) (PE); anti-FABP4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FABP4 when phosphorylated at Tyr20.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-FABP4 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr20 of human FABP4 (KLH).
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-FABP4-pY20 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-FABP4 antibody

Antibodies; Fatty Acid Binding Protein

References

1. Cabre, A., J. Lipid Res. 49(8), 1746-1751 (2008). 2. Fasshauer, M., Am. J. Hypertens. 21(5), 582-586 (2008). 3. Cabre, A., Clin. Chem. 54(1), 181-187 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4557579
• NCBI GeneID: 2167
• NCBI Accession #: NP_001433
• NCBI GenBank Nucleotide #: NM_001442.2
• UniProt Accession #: P15090; Q6IBA1
• NCBI Official Full Name: fatty acid-binding protein, adipocyte
• NCBI Official Synonym Full Names: fatty acid binding protein 4
• NCBI Official Symbol: FABP4
• NCBI Official Synonym Symbols: aP2; ALBP; AFABP; A-FABP; HEL-S-104
• NCBI Protein Information: fatty acid-binding protein, adipocyte
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP4
• UniProt Synonym Gene Names: ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_HUMAN

NCBI Description

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoplasm; lipid particle; nucleus

Molecular Function: transporter activity; fatty acid binding

Biological Process: cholesterol homeostasis; transport; white fat cell differentiation; triacylglycerol catabolic process; brown fat cell differentiation; negative regulation of protein kinase activity; cytokine production; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response