Rabbit anti-Human EZH1 Polyclonal Antibody | anti-EZH1 antibody

EZH1, ID (Histone-lysine N-methyltransferase EZH1, Enhancer Of Zeste Homolog 1, ENX-2, KIAA0388) (Biotin)

Cat. Num. AAA6475476

• Gene Names: EZH1; KMT6B
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: EZH1; Polyclonal Antibody; ID (Histone-lysine N-methyltransferase EZH1; Enhancer Of Zeste Homolog 1; ENX-2; KIAA0388) (Biotin); anti-EZH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EZH1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EZH1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1,000; WB: 1:50-1:100; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human EZH1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-EZH1 Antibody (Center) in 293 cell line lysates (35ug/lane). EZH1(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with EZH1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-EZH1 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Ogawa, M., et al., Biochim. Biophys. Acta 1395(2):151-158 (1998). 2. Abel, K.J., et al., Genomics 37(2):161-171 (1996). 3. Friedman, L.S., et al., Genomics 25(1):256-263 (1995). 4. Osborne-Lawrence, S., et al., Genomics 25(1):248-255 (1995). 5. Brody, L.C., et al., Genomics 25(1):238-247 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 19923202
• NCBI GeneID: 2145
• NCBI Accession #: NP_001982.2
• NCBI GenBank Nucleotide #: NM_001991.3
• UniProt Accession #: Q92800; O43287; Q14459; Q53XP3; A6NCH6; B4DIJ1; B4DIZ7; B4DSS2; B4E3R7
• Molecular Weight: 85,271 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase EZH1
• NCBI Official Synonym Full Names: enhancer of zeste homolog 1 (Drosophila)
• NCBI Official Symbol: EZH1
• NCBI Official Synonym Symbols: KMT6B
• NCBI Protein Information: histone-lysine N-methyltransferase EZH1; ENX-2
• UniProt Protein Name: Histone-lysine N-methyltransferase EZH1
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: EZH1
• UniProt Synonym Gene Names: KIAA0388
• UniProt Entry Name: EZH1_HUMAN

NCBI Description

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]

Uniprot Description

EZH1: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Belongs to the histone-lysine methyltransferase family. EZ subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 17q21.1-q21.3

Cellular Component: nucleoplasm; ESC/E(Z) complex

Molecular Function: chromatin binding; histone lysine N-methyltransferase activity (H3-K27 specific)

Biological Process: anatomical structure morphogenesis; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter