Rabbit anti-Human, Mouse Exportin 1 Polyclonal Antibody | anti-Exp1 antibody

Exportin 1, CT (XPO1, Chromosome Region Maintenance 1 Protein Homolog, CRM1, DKFZp686B1823, emb, Exp1, Exportin-1) (APC)

Cat. Num. AAA6475463

• Gene Names: XPO1; emb; CRM1; exp1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Exportin 1; Polyclonal Antibody; CT (XPO1; Chromosome Region Maintenance 1 Protein Homolog; CRM1; DKFZp686B1823; emb; Exp1; Exportin-1) (APC); anti-Exp1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human XPO1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-Exp1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human XPO1 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of XPO1 antibody (C-term) in mouse bladder tissue lysates (35ug/lane). XPO1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with XPO1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of widr cells using XPO1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-Exp1 antibody

Antibodies; RNA Processing

References

1. Shen, A., Neurosurgery 65(1), 153-159 (2009). 2. Dong, X., Nat. Struct. Mol. Biol. 16(5), 558-560 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4507943
• NCBI GeneID: 7514
• NCBI Accession #: NP_003391
• NCBI GenBank Nucleotide #: NM_003400
• UniProt Accession #: O14980; Q63HP8; Q68CP3; Q99433; A6NL14; A8K1K5; D6W5E2
• Molecular Weight: 123,386 Da
• NCBI Official Full Name: exportin-1
• NCBI Official Synonym Full Names: exportin 1
• NCBI Official Symbol: XPO1
• NCBI Official Synonym Symbols: emb; CRM1; exp1
• NCBI Protein Information: exportin-1; chromosome region maintenance 1 homolog; chromosome region maintenance 1 protein homolog; exportin 1 (CRM1 homolog, yeast); exportin-1 (required for chromosome region maintenance)
• UniProt Protein Name: Exportin-1
• UniProt Gene Name: XPO1
• UniProt Synonym Gene Names: CRM1; Exp1
• UniProt Entry Name: XPO1_HUMAN

Uniprot Description

Exportin-1: Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of an nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. Belongs to the exportin family.

Protein type: Karyopherin; Nuclear import; Nucleolus; Nuclear export

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: kinetochore; nucleoplasm; Cajal body; nuclear membrane; intracellular membrane-bound organelle; membrane; cytoplasm; nucleolus; nuclear envelope; cytosol; ribonucleoprotein complex; nucleus; annulate lamellae

Molecular Function: protein domain specific binding; protein binding; RNA binding; transporter activity; Ran GTPase binding; protein transporter activity; nucleocytoplasmic transporter activity

Biological Process: response to drug; ribosomal small subunit export from nucleus; mRNA transport; viral reproduction; transforming growth factor beta receptor signaling pathway; regulation of protein catabolic process; gene expression; viral infectious cycle; negative regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; protein export from nucleus; negative regulation of transforming growth factor beta receptor signaling pathway; ribosomal large subunit export from nucleus; regulation of protein export from nucleus