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Goat anti-E Coli Escherichia coli Polyclonal Antibody

Escherichia coli (E. coli) (AP)

Reactivity
E Coli
Applications
ELISA, Dot Blot, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
Escherichia coli; Polyclonal Antibody; Escherichia coli (E. coli) (AP); anti-Escherichia coli antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
E Coli
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes all E. coli species in immunogen, plus O157-H7. Recognizes more than 50 proteins by Western Blot.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Escherichia coli antibody
ELISA (EIA), Dot Blot (DB), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Heat inactivated blend of 6 different strains of E. coli: TOP 10F, HB101, JM109, BL21, DH5 alpha and K12.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Related Product Information for anti-Escherichia coli antibody
Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae-bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.
Product Categories/Family for anti-Escherichia coli antibody
References
Smith, G.P. and Gingrich, T.R., (2005). BioTechniques 39; 879-883.

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Product Notes

The Escherichia coli (Catalog #AAA6474867) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Escherichia coli (E. coli) (AP) reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's Escherichia coli can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Dot Blot (DB), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Escherichia coli for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Escherichia coli, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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