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Western Blot (WB) (Western blot analysis of ELF5 Antibody (Center) in MCF7(lane 1),T47D cell line lysates(lane 2) and mouse lung tissues(lane 3) lysates(35ug/lane). ELF5 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse ELF5 Polyclonal Antibody | anti-ELF5 antibody

ELF5, ID (ETS-related Transcription Factor Elf-5, E74-like Factor 5, Epithelium-specific Ets Transcription Factor 2, ESE-2, Epithelium-restricted ESE-1-related Ets Factor, ESE2) (PE)

Gene Names
ELF5; ESE2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Immunoaffinity chromatography.
Synonyms
ELF5; Polyclonal Antibody; ID (ETS-related Transcription Factor Elf-5; E74-like Factor 5; Epithelium-specific Ets Transcription Factor 2; ESE-2; Epithelium-restricted ESE-1-related Ets Factor; ESE2) (PE); anti-ELF5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ELF5. Species Crossreactivity: mouse.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-ELF5 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human ELF5 (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ELF5 Antibody (Center) in MCF7(lane 1),T47D cell line lysates(lane 2) and mouse lung tissues(lane 3) lysates(35ug/lane). ELF5 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ELF5 Antibody (Center) in MCF7(lane 1),T47D cell line lysates(lane 2) and mouse lung tissues(lane 3) lysates(35ug/lane). ELF5 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ELF5 (arrow) using rabbit polyclonal ELF5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ELF5 gene.)

Western Blot (WB) (Western blot analysis of ELF5 (arrow) using rabbit polyclonal ELF5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ELF5 gene.)
Product Categories/Family for anti-ELF5 antibody
References
1. Choi, Y.S., Biochem. J. 398(3), 497-507 (2006). 2. Tummala, R., J. Cell. Biochem. 97(4), 766-781 (2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29kDa
NCBI Official Full Name
ETS-related transcription factor Elf-5 isoform 1
NCBI Official Synonym Full Names
E74 like ETS transcription factor 5
NCBI Official Symbol
ELF5
NCBI Official Synonym Symbols
ESE2
NCBI Protein Information
ETS-related transcription factor Elf-5
UniProt Protein Name
ETS-related transcription factor Elf-5
UniProt Gene Name
ELF5
UniProt Synonym Gene Names
ESE2; ESE-2
UniProt Entry Name
ELF5_HUMAN

NCBI Description

The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

ELF5: Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Belongs to the ETS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p13-p12

Cellular Component: cytoplasm; nucleus

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; cell proliferation; ectoderm development; positive regulation of transcription from RNA polymerase II promoter; cell differentiation

Research Articles on ELF5

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Product Notes

The ELF5 elf5 (Catalog #AAA6473705) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ELF5, ID (ETS-related Transcription Factor Elf-5, E74-like Factor 5, Epithelium-specific Ets Transcription Factor 2, ESE-2, Epithelium-restricted ESE-1-related Ets Factor, ESE2) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ELF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:50-1:100 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ELF5 elf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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