Rabbit anti-Human, Mouse ELF5 Polyclonal Antibody | anti-ELF5 antibody

ELF5, ID (ETS-related Transcription Factor Elf-5, E74-like Factor 5, Epithelium-specific Ets Transcription Factor 2, ESE-2, Epithelium-restricted ESE-1-related Ets Factor, ESE2) (Azide free) (HRP)

Cat. Num. AAA6473699

• Gene Names: ELF5; ESE2
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ELF5; Polyclonal Antibody; ID (ETS-related Transcription Factor Elf-5; E74-like Factor 5; Epithelium-specific Ets Transcription Factor 2; ESE-2; Epithelium-restricted ESE-1-related Ets Factor; ESE2) (Azide free) (HRP); anti-ELF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ELF5. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ELF5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human ELF5 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ELF5 Antibody (Center) in MCF7(lane 1),T47D cell line lysates(lane 2) and mouse lung tissues(lane 3) lysates(35ug/lane). ELF5 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ELF5 (arrow) using rabbit polyclonal ELF5 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ELF5 gene.)

Product Categories/Family for anti-ELF5 antibody

Antibodies; Transcription Factors

References

1. Choi, Y.S., Biochem. J. 398(3), 497-507 (2006). 2. Tummala, R., J. Cell. Biochem. 97(4), 766-781 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 38201717
• NCBI GeneID: 2001
• NCBI Accession #: NP_938195
• NCBI GenBank Nucleotide #: NM_198381.1
• UniProt Accession #: Q9UKW6
• Molecular Weight: 29kDa
• NCBI Official Full Name: ETS-related transcription factor Elf-5 isoform 1
• NCBI Official Synonym Full Names: E74 like ETS transcription factor 5
• NCBI Official Symbol: ELF5
• NCBI Official Synonym Symbols: ESE2
• NCBI Protein Information: ETS-related transcription factor Elf-5
• UniProt Protein Name: ETS-related transcription factor Elf-5
• Protein Family: ETS-related transcription factor
• UniProt Gene Name: ELF5
• UniProt Synonym Gene Names: ESE2; ESE-2
• UniProt Entry Name: ELF5_HUMAN

NCBI Description

The protein encoded by this gene is a member of an epithelium-specific subclass of the Ets transcritpion factor family. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

ELF5: Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Belongs to the ETS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p13-p12

Cellular Component: cytoplasm; nucleus

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; cell proliferation; ectoderm development; positive regulation of transcription from RNA polymerase II promoter; cell differentiation