Rabbit anti-Human EHD3 Polyclonal Antibody | anti-EHD3 antibody

EHD3, ID (EH Domain-containing Protein 3, PAST Homolog 3, EHD2, PAST3) (AP)

Cat. Num. AAA6473618

• Gene Names: EHD3; PAST3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: EHD3; Polyclonal Antibody; ID (EH Domain-containing Protein 3; PAST Homolog 3; EHD2; PAST3) (AP); anti-EHD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EHD3.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-EHD3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human EHD3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EHD3 Antibody (Center) in CEM cell line lysates (35ug/lane). EHD3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human kidney carcinoma reacted with EHD3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of 293 cells using EHD3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EHD3 antibody

Antibodies; Transport Proteins

References

1. Pohl U., Smith J.S.Genomics 63:255-262(2000). 2. George M., Ying G.BMC Cell Biol. 8:3-3(2007). 3. Sharma M., Naslavsky N.Traffic 9:995-1018(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 7657056
• NCBI GeneID: 30845
• NCBI Accession #: NP_055415
• NCBI GenBank Nucleotide #: NM_014600.2
• UniProt Accession #: Q9NZN3; Q8N514; Q9NZB3; B4DFR5; D6W574
• Molecular Weight: 61 kDa
• NCBI Official Full Name: EH domain-containing protein 3
• NCBI Official Synonym Full Names: EH domain containing 3
• NCBI Official Symbol: EHD3
• NCBI Official Synonym Symbols: PAST3
• NCBI Protein Information: EH domain-containing protein 3
• UniProt Protein Name: EH domain-containing protein 2
• Protein Family: EH domain-containing protein
• UniProt Gene Name: EHD2
• UniProt Entry Name: EHD2_HUMAN

Uniprot Description

EHD2: Plays a role in membrane reorganization in response to nucleotide hydrolysis. Binds to liposomes and deforms them into tubules. Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4. Required for normal fusion of myoblasts to skeletal muscle myotubes. Binds ATP; does not bind GTP.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: caveola; cytosol; endosome membrane; extrinsic to membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome membrane

Molecular Function: ATP binding; calcium ion binding; GTP binding; hydrolase activity; nucleic acid binding; protein binding; protein domain specific binding

Biological Process: blood coagulation; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; endocytosis; metabolic process