Rabbit anti-Human, Mouse CYP1A1 Polyclonal Antibody | anti-CYP1A1 antibody

CYP1A1, CT (Cytochrome P450 1A1, Cytochrome P450-P1, Cytochrome P450 Form 6, Cytochrome P450-C) (PE)

Cat. Num. AAA6471523

• Gene Names: CYP1A1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CYP1A1; Polyclonal Antibody; CT (Cytochrome P450 1A1; Cytochrome P450-P1; Cytochrome P450 Form 6; Cytochrome P450-C) (PE); anti-CYP1A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP1A1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CYP1A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CYP1A1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CYP1A1 Antibody (Center) in mouse lung tissue lysates (35ug/lane). CYP1A1 (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CYP1A1 Antibody (Center) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

Immunohistochemistry (IHC)

(CYP1A1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYP1A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-CYP1A1 antibody

Antibodies; Enzymes; Cytochrome

References

1. Delpisheh, A., Eur. J. Obstet. Gynecol. Reprod. Biol. 143(1), 38-42 (2009). 2. Zhuo, W., Cancer Invest. 27(1), 86-95 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503199
• NCBI GeneID: 1543
• NCBI Accession #: NP_000490
• NCBI GenBank Nucleotide #: NM_000499.3
• UniProt Accession #: P04798; Q53G18; A4F3V9; A4F3W0
• Molecular Weight: 18,441 Da
• NCBI Official Full Name: cytochrome P450 1A1
• NCBI Official Synonym Full Names: cytochrome P450, family 1, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP1A1
• NCBI Official Synonym Symbols: AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX
• NCBI Protein Information: cytochrome P450 1A1
• Protein Family: Cytochrome

NCBI Description

This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq, Jul 2008]