Rabbit anti-Human Cyclin D1 Polyclonal Antibody | anti-CCND1 antibody

Cyclin D1, ID (CCND1 Protein, Cyclin D1, Isoform CRA_c, cDNA, FLJ93625, Homo Sapiens Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1) (CCND1),mRNA, Cyclin D1, PRAD1: Parathyroid Adenomatosis 1, CCND1) (FITC)

Cat. Num. AAA6471210

• Gene Names: CCND1; BCL1; PRAD1; U21B31; D11S287E
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Cyclin D1; Polyclonal Antibody; ID (CCND1 Protein; Isoform CRA_c; cDNA; FLJ93625; Homo Sapiens Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1) (CCND1); mRNA; PRAD1: Parathyroid Adenomatosis 1; CCND1) (FITC); anti-CCND1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CCND1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CCND1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human CCND1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of CCND1 (arrow) using rabbit polyclonal CCND1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCND1 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-CCND1 antibody

Antibodies; Cyclin

References

1. He, Y.Y., Cancer Res. 68(10), 3752-3758 (2008). 2. Marsit, C.J., Clin. Cancer Res. 14(8), 2371-2377 (2008). 3. Caldon, C.E., Cancer Res. 68(8), 3026-3036 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 16950655
• NCBI GeneID: 595
• NCBI Accession #: NP_444284
• NCBI GenBank Nucleotide #: NM_053056
• UniProt Accession #: Q6FI00; P24385
• Molecular Weight: 33,729 Da
• NCBI Official Full Name: G1/S-specific cyclin-D1
• NCBI Official Synonym Full Names: cyclin D1
• NCBI Official Symbol: CCND1
• NCBI Official Synonym Symbols: BCL1; PRAD1; U21B31; D11S287E
• NCBI Protein Information: G1/S-specific cyclin-D1; B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1 oncogene; PRAD1 oncogene
• UniProt Protein Name: CCND1 protein
• Protein Family: G1/S-specific cyclin
• UniProt Gene Name: CCND1
• UniProt Entry Name: Q6FI00_HUMAN

Uniprot Description

CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Activator; Oncoprotein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; tight junction; transcriptional repressor complex; membrane; cyclin-dependent protein kinase holoenzyme complex; intracellular; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; histone deacetylase binding; protein complex binding; cyclin-dependent protein kinase regulator activity; protein kinase binding; transcription factor binding; transcription corepressor activity; protein kinase activity

Biological Process: fat cell differentiation; lactation; G1 DNA damage checkpoint; establishment and/or maintenance of chromatin architecture; re-entry into mitotic cell cycle; negative regulation of Wnt receptor signaling pathway; positive regulation of cyclin-dependent protein kinase activity; positive regulation of mammary gland epithelial cell proliferation; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; response to magnesium ion; response to vitamin E; Leydig cell differentiation; response to iron ion; response to corticosterone stimulus; response to X-ray; response to drug; Notch signaling pathway; organ regeneration; transcription, DNA-dependent; unfolded protein response; mammary gland epithelial cell proliferation; response to organic nitrogen; liver development; response to ethanol; cell division; response to estrogen stimulus; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; response to calcium ion; response to DNA damage stimulus; G1/S transition of mitotic cell cycle

Disease: Von Hippel-lindau Syndrome; Myeloma, Multiple