Western Blot (WB)
((0.1ug/ml) staining of Rat Cortical Neuron lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. Ya-Jing Mi and Dr. Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.)
Goat SRGAP2 Polyclonal Antibody | anti-SRGAP2 antibody
Goat anti-SRGAP2 Antibody
Western Blot: Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentration: 0.1-0.3ug/ml.
Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).
Western Blot (WB)
((0.1ug/ml) staining of Rat Cortical Neuron lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. Ya-Jing Mi and Dr. Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.)
Western Blot (WB)
((0.1ug/ml) staining of Rat Cortical Neuron lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data kindly provided by Ms. Ya-Jing Mi and Dr. Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.)
Immunofluorescence (IF)
(HEK293 overexpressing Human srGAP2 and probed at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr. Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.)
Immunofluorescence (IF)
(HEK293 overexpressing Human srGAP2 and probed at 2.5ug/ml in the right panel. Data kindly provided by Ms. Ya-Jing Mi and Dr. Wei-Lin Jin, Institute of Neurosciences, Shanghai Jiao Tong University.)
NCBI and Uniprot Product Information
NCBI Description
This locus encodes a member of the SLIT-ROBO Rho GTPase activating protein family. The encoded protein stimulates GTPase activity of Rac1, and plays a role in cortical neuron development. This locus has several paralogs on human chromosome 1 resulting from segmental duplication. While this locus itself is conserved among various species, the paralogs are found only in the genus Homo, and not in the genomes of non-human great apes. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Jul 2014]
Uniprot Description
SRGAP2: RAC1 GTPase activating protein (GAP) that binds and deforms membranes, and regulates actin dynamics to regulate cell migration and differentiation. Plays an important role in different aspects of neuronal morphogenesis and migration mainly during development of the cerebral cortex. This includes the biogenesis of neurites, where it is required for both axons and dendrites outgrowth, and the maturation of the dendritic spines. Also stimulates the branching of the leading process and negatively regulates neuron radial migration in the cerebral cortex. Its interaction and inhibition by SRGAP2C reduces the rate of spine maturation, alters dendritic spine morphology and density and indirectly increases neuronal migration. It may have implications for cognition, learning and memory. In non-neuronal cells, it may also play a role in cell migration by regulating the formation of lamellipodia and filopodia. A chromosomal aberration disrupting SRGAP2 has been found in a patient with early infantile epileptic encephalopathy. Balanced translocation t(1;9)(q32;q13).
Protein type: GAPs; Mitochondrial; GAPs, Rac/Rho; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: cell junction; cytoplasm; cytosol; lamellipodium; nucleus; phagocytic vesicle; plasma membrane; postsynaptic density; postsynaptic membrane
Molecular Function: GTPase activator activity; protein binding; protein homodimerization activity; Rac GTPase binding
Biological Process: actin filament severing; cell proliferation; extension of a leading process involved in cell locomotion in cerebral cortex radial glia guided migration; filopodium formation; neurite morphogenesis; positive regulation of GTPase activity; regulation of small GTPase mediated signal transduction; signal transduction
Research Articles on SRGAP2
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Product Notes
The SRGAP2 srgap2 (Catalog #AAA422275) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SRGAP2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SRGAP2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunofluorescence (IF). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentration: 0.1-0.3ug/ml. Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF). Researchers should empirically determine the suitability of the SRGAP2 srgap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KQEDRQTPRS PDST. It is sometimes possible for the material contained within the vial of "SRGAP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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