Rabbit anti-Human CDC6 Polyclonal Antibody | anti-CDC6 antibody

CDC6, phosphorylated Ser54 (Cell Division Control Protein 6 Homolog, CDC6-related Protein, p62(cdc6), HsCDC6, Cdc18-related Protein, HsCDC18, CDC18L) (Azide free) (HRP)

Cat. Num. AAA6468581

• Gene Names: CDC6; CDC18L; HsCDC6; HsCDC18
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CDC6; Polyclonal Antibody; phosphorylated Ser54 (Cell Division Control Protein 6 Homolog; CDC6-related Protein; p62(cdc6); HsCDC6; Cdc18-related Protein; HsCDC18; CDC18L) (Azide free) (HRP); anti-CDC6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cdc6 phosphorylated at Ser55.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CDC6 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser54 of human Cdc6 (KLH).
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Phospho-CDC6-S54 Pab is used in Western blot to detect Phospho-CDC6-S54 in Ramos tissue lysate)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-CDC6 antibody

Antibodies; Cell Division Cycle (CDC)

References

1. Alexandrow, M.G., et al., Mol. Cell. Biol. 24(4):1614-1627 (2004). 2. Yim, H., et al., Mol. Biol. Cell 14(10):4250-4259 (2003). 3. Clay-Farrace, L., et al., EMBO J. 22(3):704-712 (2003). 4. Pelizon, C., et al., EMBO Rep. 3(8):780-784 (2002). 5. Bermejo, R., et al., Mol. Biol. Cell 13(11):3989-4000 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4502703
• NCBI GeneID: 990
• NCBI Accession #: NP_001245
• NCBI GenBank Nucleotide #: NM_001254
• UniProt Accession #: Q99741; Q8TB30
• Molecular Weight: 62,720 Da
• NCBI Official Full Name: cell division control protein 6 homolog
• NCBI Official Synonym Full Names: cell division cycle 6
• NCBI Official Symbol: CDC6
• NCBI Official Synonym Symbols: CDC18L; HsCDC6; HsCDC18
• NCBI Protein Information: cell division control protein 6 homolog; CDC6 cell division cycle 6 homolog; CDC6-related protein; cdc18-related protein; p62(cdc6)
• UniProt Protein Name: Cell division control protein 6 homolog
• Protein Family: Cell division control protein
• UniProt Gene Name: CDC6
• UniProt Synonym Gene Names: CDC18L; HsCdc18; HsCDC6
• UniProt Entry Name: CDC6_HUMAN

Uniprot Description

CDC6: Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. Interacts with PCNA, ORC1L, cyclin-CDK and HUWE1. Belongs to the CDC6/cdc18 family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: nucleoplasm; Golgi apparatus; spindle pole; cytoplasm; spindle midzone; nucleus; cytosol

Molecular Function: protein binding; nucleotide binding; chromatin binding; kinase binding; ATP binding

Biological Process: positive regulation of cytokinesis; mitosis; traversing start control point of mitotic cell cycle; regulation of mitotic metaphase/anaphase transition; positive regulation of chromosome segregation; G1/S-specific transcription in mitotic cell cycle; negative regulation of cell proliferation; cell division; negative regulation of DNA replication; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; DNA replication checkpoint; DNA replication; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 5