Rabbit anti-Human, Mouse CA4 Polyclonal Antibody | anti-CA4 antibody

CA4, CT (Carbonic Anhydrase 4, Carbonic Anhydrase IV, CA-IV, Carbonate Dehydratase IV) (MaxLight 405)

Cat. Num. AAA6467014

• Gene Names: CA4; CAIV; Car4; RP17
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: CA4; Polyclonal Antibody; CT (Carbonic Anhydrase 4; Carbonic Anhydrase IV; CA-IV; Carbonate Dehydratase IV) (MaxLight 405); anti-CA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CA4. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-CA4 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CA4 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CA4 Antibody (C-term) in mouse lung tissue lysates (35ug/lane). CA4 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CA4 antibody

Antibodies; Enzymes

References

1. Okuyama T., Sato S.Proc. Natl. Acad. Sci. USA 89:1315-1319(1992). 2. Okuyama T.Genomics 16:678-684(1993). 3. Yang Z., Alvarez B.V.Hum. Mol. Genet. 14:255-265(2005). 4. Okuyama T.Arch. Biochem. Biophys. 320:315-322(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4502519
• NCBI GeneID: 762
• NCBI Accession #: NP_000708
• NCBI GenBank Nucleotide #: NM_000717
• UniProt Accession #: P22748
• Molecular Weight: 34kDa
• NCBI Official Full Name: carbonic anhydrase 4 preproprotein
• NCBI Official Synonym Full Names: carbonic anhydrase 4
• NCBI Official Symbol: CA4
• NCBI Official Synonym Symbols: CAIV; Car4; RP17
• NCBI Protein Information: carbonic anhydrase 4
• UniProt Protein Name: Carbonic anhydrase 4
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA4
• UniProt Synonym Gene Names: CA-IV
• UniProt Entry Name: CAH4_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008]

Uniprot Description

CA4: Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. Defects in CA4 are the cause of retinitis pigmentosa type 17 (RP17). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP17 inheritance is autosomal dominant. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; Membrane protein, GPI anchor; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 17q23

Cellular Component: Golgi apparatus; cell surface; sarcoplasmic reticulum; rough endoplasmic reticulum; basolateral plasma membrane; brush border membrane; secretory granule membrane; ER-Golgi intermediate compartment; trans-Golgi network; anchored to external side of plasma membrane; membrane; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; sarcolemma

Molecular Function: carbonate dehydratase activity; protein binding; zinc ion binding

Biological Process: response to drug; response to steroid hormone stimulus; bicarbonate transport; organ development; one-carbon compound metabolic process

Disease: Retinitis Pigmentosa 17