Rabbit anti-Human Calmodulin Polyclonal Antibody | anti-CAM antibody

Calmodulin, CT (CaM, CALM, CAM1, CAM) (Biotin)

Cat. Num. AAA6466703

• Gene Names: CALM1; caM; CAMI; PHKD; CPVT4; DD132; CALML2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Calmodulin; Polyclonal Antibody; CT (CaM; CALM; CAM1; CAM) (Biotin); anti-CAM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Calmodulin.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CAM antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human Calmodulin (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CALM1 Antibody (C-term) in Hela cell line lysates (35ug/lane). CALM1 (arrow) was detected using the purified Pab.(2ug/ml))

Flow Cytometry (FC/FACS)

(CALM1 Antibody (C-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(CALM1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CALM1 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-CAM antibody

Antibodies; Calmodulin

References

1. Zhao, D., et.al., Zhonghua Yi Xue Za Zhi 88(35), 2452-2456 (2008). 2. Martins-de-Souza, D., et.al., J. Neural Transm. 116(3), 275-289 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5901912
• NCBI GeneID: 801
• NCBI Accession #: NP_008819
• NCBI GenBank Nucleotide #: NM_006888
• UniProt Accession #: P62158; P02593; P70667; P99014; Q13942; Q53S29; Q61379; Q61380; Q96HK3
• Molecular Weight: 16,838 Da
• NCBI Official Full Name: calmodulin
• NCBI Official Synonym Full Names: calmodulin 1 (phosphorylase kinase, delta)
• NCBI Official Symbol: CALM1
• NCBI Official Synonym Symbols: caM; CAMI; PHKD; CPVT4; DD132; CALML2
• NCBI Protein Information: calmodulin; phosphorylase kinase, delta subunit; prepro-calmodulin 1
• UniProt Protein Name: Calmodulin
• Protein Family: Calmodulin
• UniProt Gene Name: CALM1
• UniProt Synonym Gene Names: CALM; CAM; CAM1; CaM
• UniProt Entry Name: CALM_HUMAN

Uniprot Description

Calmodulin: a calcium-binding small regulatory protein that mediates the control of a large number of enzymes by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Has four functional calcium-binding domains. Phosphorylation and ubiquitination result in a decreased activity.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: nucleoplasm; spindle pole; centrosome; sarcomere; growth cone; spindle microtubule; cytoplasm; plasma membrane; extracellular region; cytosol; nucleus; vesicle

Molecular Function: protein domain specific binding; nitric-oxide synthase regulator activity; titin binding; calcium ion binding; calcium-dependent protein binding; protein kinase binding; protein serine/threonine kinase activator activity; protein binding; N-terminal myristoylation domain binding; phospholipase binding; thioesterase binding; type 3 metabotropic glutamate receptor binding; phosphoinositide 3-kinase binding; nitric-oxide synthase binding; adenylate cyclase binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; phototransduction, visible light; inositol phosphate metabolic process; nerve growth factor receptor signaling pathway; pathogenesis; signal transduction; nitric oxide metabolic process; positive regulation of protein amino acid dephosphorylation; synaptic transmission; substantia nigra development; platelet degranulation; muscle contraction; positive regulation of cyclic nucleotide metabolic process; response to corticosterone stimulus; epidermal growth factor receptor signaling pathway; mitochondrion organization and biogenesis; platelet activation; fibroblast growth factor receptor signaling pathway; glycogen catabolic process; regulation of heart rate; positive regulation of nitric-oxide synthase activity; organelle organization and biogenesis; adenylate cyclase activation; response to amphetamine; glucose metabolic process; positive regulation of protein amino acid autophosphorylation; regulation of cytokinesis; rhodopsin mediated signaling; G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling; phospholipase C activation; carbohydrate metabolic process; innate immune response; positive regulation of cyclic-nucleotide phosphodiesterase activity; detection of calcium ion; regulation of nitric-oxide synthase activity; blood coagulation; response to calcium ion; vascular endothelial growth factor receptor signaling pathway

Disease: Long Qt Syndrome 14; Ventricular Tachycardia, Catecholaminergic Polymorphic, 4