Rabbit anti-Human, Mouse BTBD1 Polyclonal Antibody | anti-BTBD1 antibody

BTBD1, ID (BTB/POZ Domain-containing Protein 1, Hepatitis C Virus NS5A-transactivated Protein 8, HCV NS5A-transactivated Protein 8, NS5ATP8, C15orf1) (MaxLight 405)

Cat. Num. AAA6466078

• Gene Names: BTBD1; C15orf1; NS5ATP8
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: BTBD1; Polyclonal Antibody; ID (BTB/POZ Domain-containing Protein 1; Hepatitis C Virus NS5A-transactivated Protein 8; HCV NS5A-transactivated Protein 8; NS5ATP8; C15orf1) (MaxLight 405); anti-BTBD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BTBD1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-BTBD1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human BTBD1 (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BTBD1 antibody (Center) in 293 cell line lysates (35ug/lane). BTBD1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of BTBD1 antibody (Center) in mouse cerebellum tissue lysates (35ug/lane). BTBD1 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(BTBD1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-BTBD1 antibody

Antibodies; Proteins

References

1. Pisani, D.F., Cabane, C. Cell Death Differ. 11(11), 1157-1165 (2004). 2. Xu, L., Yang, L. Exp. Cell Res. 288(1), 84-93 (2003). 3. Xu, L., Yang, L. BMC Genomics 3(1), 1 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 13376848
• NCBI GeneID: 53339
• NCBI Accession #: NP_079514
• NCBI GenBank Nucleotide #: NM_025238
• UniProt Accession #: Q9H0C5
• Molecular Weight: 53kDa
• NCBI Official Full Name: BTB/POZ domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: BTB domain containing 1
• NCBI Official Symbol: BTBD1
• NCBI Official Synonym Symbols: C15orf1; NS5ATP8
• NCBI Protein Information: BTB/POZ domain-containing protein 1
• UniProt Protein Name: BTB/POZ domain-containing protein 1
• UniProt Gene Name: BTBD1
• UniProt Synonym Gene Names: C15orf1; NS5ATP8; HCV NS5A-transactivated protein 8
• UniProt Entry Name: BTBD1_HUMAN

NCBI Description

The C-terminus of the protein encoded by this gene binds topoisomerase I. The N-terminus contains a proline-rich region and a BTB/POZ domain (broad-complex, Tramtrack and bric a brac/Pox virus and Zinc finger), both of which are typically involved in protein-protein interactions. Subcellularly, the protein localizes to cytoplasmic bodies. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]