Rabbit anti-Human, Mouse BRD3 Polyclonal Antibody | anti-BRD3 antibody

BRD3, CT (Bromodomain-containing Protein 3, RING3-like Protein, KIAA0043, RING3L) (AP)

Cat. Num. AAA6466018

• Gene Names: BRD3; ORFX; RING3L
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: BRD3; Polyclonal Antibody; CT (Bromodomain-containing Protein 3; RING3-like Protein; KIAA0043; RING3L) (AP); anti-BRD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BRD3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-BRD3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human BRD3 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-BRD3 Pab is used in Western blot to detect BRD3 in mouse brain tissue lysate (Lane 1) and Saos-2 cell lysate (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-BRD3 antibody

Antibodies; Proteins

References

1. Kaneko, H., et al., J Investig Allergol Clin Immunol 12(2):86-90 (2002). 2. Thorpe, K.L., et al., Immunogenetics 44(5):391-396 (1996). 3. Thorpe, K.L., et al., Gene 200(1-2), 177-183 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 11067749
• NCBI GeneID: 8019
• NCBI Accession #: NP_031397.1
• NCBI GenBank Nucleotide #: NM_007371.3
• UniProt Accession #: Q15059; Q4G5Y3; Q5T1R7; Q8N5M3; Q92645; B1APD9
• Molecular Weight: 79,542 Da
• NCBI Official Full Name: bromodomain-containing protein 3
• NCBI Official Synonym Full Names: bromodomain containing 3
• NCBI Official Symbol: BRD3
• NCBI Official Synonym Symbols: ORFX; RING3L
• NCBI Protein Information: bromodomain-containing protein 3; RING3-like protein; bromodomain-containing 3
• UniProt Protein Name: Bromodomain-containing protein 3
• Protein Family: Bromodomain-containing protein
• UniProt Gene Name: BRD3
• UniProt Synonym Gene Names: KIAA0043; RING3L
• UniProt Entry Name: BRD3_HUMAN

NCBI Description

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

BRD3: Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets. Regulates transcription of the CCND1 gene. A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUT which produces a BRD3-NUT fusion protein. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, atypical; ATYPICAL group; BRD family

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; chromatin modification