Rabbit anti-Human BRSK2 Polyclonal Antibody | anti-BRSK2 antibody

BRSK2, CT (BR Serine/Threonine-protein Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, STK29, HUSSY-12) (AP)

Cat. Num. AAA6465753

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: BRSK2; Polyclonal Antibody; CT (BR Serine/Threonine-protein Kinase 2; Serine/Threonine-protein Kinase 29; Serine/Threonine-protein Kinase SAD-A; C11orf7; PEN11B; STK29; HUSSY-12) (AP); anti-BRSK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human STK29.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 736

• Applicable Applications for anti-BRSK2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human STK29 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-STK29 Pab in HL60 cell line lysate (35ug/lane). STK29(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-BRSK2 antibody

Antibodies; Protein Kinases

References

1. J. Biol. Chem. 279: 31164-31170, 2004. 2. J. Hum. Genet. 44:1-9(1999). 3. J. Hum. Genet. 43: 283-284, 1998.

NCBI and Uniprot Product Information

• NCBI GI #: 33187744
• UniProt Accession #: Q8IWQ3
• Molecular Weight: 81.607
• NCBI Official Full Name: putative serine/threonine protein kinase variant C
• UniProt Protein Name: Serine/threonine-protein kinase BRSK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: BRSK2
• UniProt Synonym Gene Names: C11orf7; PEN11B; SADA; STK29; BR serine/threonine-protein kinase 2
• UniProt Entry Name: BRSK2_HUMAN

Uniprot Description

BRSK2: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. BRSK1 and BRSK2 are required for nueronal polarization. Expressed in the brain and at low levels in the testis. Four alternatively spliced isoforms have been described.

Protein type: EC 2.7.11.1; EC 2.7.11.26; Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMKL family; BRSK subfamily

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: centrosome; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein serine/threonine kinase activity; tau-protein kinase activity; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: neuron differentiation; mitosis; peptidyl-serine phosphorylation; exocytosis; axonogenesis; cell division; establishment of cell polarity; actin cytoskeleton reorganization; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation