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Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NAT8 protein using NAT8 antibody)

Mouse NAT8 Monoclonal Antibody | anti-NAT8 antibody

NAT8 antibody

Gene Names
NAT8; GLA; CML1; CCNAT; Hcml1; ATase2; TSC501; TSC510
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
NAT8 antibody was purified by affinity chromatography.
Synonyms
NAT8; Monoclonal Antibody; NAT8 antibody; Monoclonal NAT8; Anti-NAT8; N-acetyltransferase 8; GLA; TSC501; Hcml1; Camello-like protein 1; Cml1; TSC510; Kidney-and liver-specific gene product; NAT 8; NAT-8; anti-NAT8 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6H3
Purity/Purification
NAT8 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
227
Applicable Applications for anti-NAT8 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:100
IHC: 1:50
WB: 1:500
Biological Significance
NAT8 is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar proteinin Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway.
Cross-Reactivity
Human
Immunogen
NAT8 antibody was raised in mouse using a protein expressed in 293T cell transfected with human NAT8 expression vector as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NAT8 protein using NAT8 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NAT8 protein using NAT8 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NAT8 protein (Right) or empty vector (Left) detected with NAT8 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NAT8 protein (Right) or empty vector (Left) detected with NAT8 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NAT8 protein in paraffin embedded Adenocarcinoma of Human colon tissue using NAT8 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of NAT8 protein in paraffin embedded Adenocarcinoma of Human colon tissue using NAT8 antibody)
Related Product Information for anti-NAT8 antibody
Mouse monoclonal NAT8 antibody
Product Categories/Family for anti-NAT8 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
25,619 Da
NCBI Official Full Name
NAT8, partial
NCBI Official Synonym Full Names
N-acetyltransferase 8 (GCN5-related, putative)
NCBI Official Symbol
NAT8
NCBI Official Synonym Symbols
GLA; CML1; CCNAT; Hcml1; ATase2; TSC501; TSC510
NCBI Protein Information
N-acetyltransferase 8
UniProt Protein Name
N-acetyltransferase 8
Protein Family
UniProt Gene Name
NAT8
UniProt Synonym Gene Names
ATase2; CCNAT
UniProt Entry Name
NAT8_HUMAN

NCBI Description

This gene, isolated using the differential display method to detect tissue-specific genes, is specifically expressed in kidney and liver. The encoded protein shows amino acid sequence similarity to N-acetyltransferases. A similar protein in Xenopus affects cell adhesion and gastrulation movements, and may be localized in the secretory pathway. A highly similar paralog is found in a cluster with this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

NAT8: Plays a role in regulation of gastrulation. Belongs to the camello family.

Protein type: Acetyltransferase; Cell adhesion; EC 2.3.1.80; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p13.2

Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; integral to membrane; ER-Golgi intermediate compartment

Molecular Function: lysine N-acetyltransferase activity; cysteine-S-conjugate N-acetyltransferase activity; protein binding

Biological Process: response to drug; glutathione metabolic process; beta-amyloid metabolic process; peptidyl-lysine N6-acetylation; negative regulation of apoptosis

Research Articles on NAT8

Similar Products

Product Notes

The NAT8 nat8 (Catalog #AAA832189) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NAT8 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:100 IHC: 1:50 WB: 1:500. Researchers should empirically determine the suitability of the NAT8 nat8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAT8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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