Rabbit anti-Human, Mouse Bone Morphogenic Protein Receptor 1A Polyclonal Antibody | anti-BMPR-1A antibody

Bone Morphogenic Protein Receptor 1A (CD292, BMPR-1A, Bone Morphogenetic Protein Receptor Type IA, Serine Threonine Protein Kinase Receptor R5, SKR5, Activin Receptor-like Kinase 3, ALK-3, ACVRLK3) (Azide free) (HRP)

Cat. Num. AAA6465644

• Gene Names: BMPR1A; ALK3; SKR5; CD292; ACVRLK3; 10q23del
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Bone Morphogenic Protein Receptor 1A; Polyclonal Antibody; Bone Morphogenic Protein Receptor 1A (CD292; BMPR-1A; Bone Morphogenetic Protein Receptor Type IA; Serine Threonine Protein Kinase Receptor R5; SKR5; Activin Receptor-like Kinase 3; ALK-3; ACVRLK3) (Azide free) (HRP); anti-BMPR-1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bone Morphogenic Protein Receptor 1A (BMPR-1A). Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BMPR-1A antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: ELISA: 1:1000; WB: 1:100-1:500 to detect BMPR1A in Hela cell lysate.; Applications are based on unconjugated antibody.; IHC: 1:50-1:100. Formalin-fixed and paraffin-embedded human cancer tissue was stained followed by peroxidase-conjugate secondary antibody and DAB staining.
• Immunogen: Synthetic peptide corresponding to a portion within aa 173-202 from the central region of human BMPR1A, conjugated to KLH. MW: 60198D
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BMPR1A-C180 western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BMPR1A antibody detected the BMPR1A protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-BMPR-1A antibody

Antibodies; Receptors; Growth Factor

References

1. Zhou, X.-P., et al., Am. J. Hum. Genet. 69(4): 704-711 (2001). 2. Howe, J.R., et al., Nat. Genet. 28(2): 184-187 (2001). 3. ten Dijke, P., et al., Oncogene 8(10): 2879-2887 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 41349437
• NCBI GeneID: 657
• NCBI Accession #: NP_004320.2
• NCBI GenBank Nucleotide #: NM_004329.2
• UniProt Accession #: P36894; Q8NEN8; A8K6U9
• Molecular Weight: 60,198 Da
• NCBI Official Full Name: bone morphogenetic protein receptor type-1A
• NCBI Official Synonym Full Names: bone morphogenetic protein receptor, type IA
• NCBI Official Symbol: BMPR1A
• NCBI Official Synonym Symbols: ALK3; SKR5; CD292; ACVRLK3; 10q23del
• NCBI Protein Information: bone morphogenetic protein receptor type-1A; ALK-3; BMPR-1A; BMP type-1A receptor; activin receptor-like kinase 3; activin A receptor, type II-like kinase 3; serine/threonine-protein kinase receptor R5
• UniProt Protein Name: Bone morphogenetic protein receptor type-1A
• UniProt Gene Name: BMPR1A
• UniProt Synonym Gene Names: ACVRLK3; ALK3; BMP type-1A receptor; BMPR-1A; ALK-3; SKR5
• UniProt Entry Name: BMR1A_HUMAN

NCBI Description

The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. [provided by RefSeq, Jul 2008]

Uniprot Description

BMPR1A: a serine/threonine-protein kinase receptor for Bone morphogenetic protein-2 and -4 (BMP-2 and BMP-4). Defects in BMPR1A are a cause of juvenile polyposis syndrome (JPS) and Cowden disease (CD), a cancer syndrome characterized by multiple hamartomas and by a high risk for breast, thyroid and endometriel cancers.

Protein type: Kinase, protein; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; Membrane protein, integral; TKL group; STKR family; Type1 subfamily

Chromosomal Location of Human Ortholog: 10q22.3

Cellular Component: cell soma; dendrite; plasma membrane; integral to membrane; caveola

Molecular Function: transforming growth factor beta receptor activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; metal ion binding; SMAD binding; transmembrane receptor protein serine/threonine kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: neural plate mediolateral pattern formation; transcription from RNA polymerase II promoter; developmental growth; hindlimb morphogenesis; neural crest cell development; positive regulation of transcription, DNA-dependent; mesendoderm development; paraxial mesoderm structural organization; dorsal/ventral axis specification; palate development; protein amino acid phosphorylation; negative regulation of neurogenesis; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of mesenchymal cell proliferation; ectoderm development; Mullerian duct regression; somitogenesis; in utero embryonic development; lateral mesoderm development; stem cell maintenance; positive regulation of bone mineralization; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; mesoderm formation; pituitary gland development; cartilage development; embryonic organ development; immune response; embryonic digit morphogenesis; regulation of lateral mesodermal cell fate specification; positive regulation of epithelial cell proliferation; lung development

Disease: Juvenile Polyposis Syndrome; Polyposis Syndrome, Hereditary Mixed, 2