Rabbit anti-Human Bmp1 Polyclonal Antibody | anti-Bmp1 antibody

Bmp1, CT (Bone Morphogenetic Protein 1, BMP-1, Procollagen C-proteinase, PCP, Mammalian Tolloid Protein, mTld, PCOLC) (Biotin)

Cat. Num. AAA6465485

• Gene Names: BMP1; PCP; TLD; OI13; PCP2; PCOLC
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Bmp1; Polyclonal Antibody; CT (Bone Morphogenetic Protein 1; BMP-1; Procollagen C-proteinase; PCP; Mammalian Tolloid Protein; mTld; PCOLC) (Biotin); anti-Bmp1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Bmp1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-Bmp1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human Bmp1 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Bmp1 Antibody (R972) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Bmp1 antibody detected the Bmp1 protein (arrow).)

Product Categories/Family for anti-Bmp1 antibody

Antibodies; Growth Factors; Cytokines

References

1. Leighton, M., et al., J. Biol. Chem. 278(20):18478-18484 (2003). 2. Hartigan, N., et al., J. Biol. Chem. 278(20):18045-18049 (2003). 3. Garrigue-Antar, L., et al., J. Biol. Chem. 277(45):43327-43334 (2002). 4. Rattenholl, A., et al., J. Biol. Chem. 277(29):26372-26378 (2002). 5. Janitz, M., et al., J. Mol. Med. 76(2):141-146 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 5453579
• NCBI GeneID: 649
• NCBI Accession #: NP_006120.1
• NCBI GenBank Nucleotide #: NM_006129.4
• UniProt Accession #: P13497; Q13292; Q13872; Q14874; Q99421; Q99422; Q99423; Q9UL38; A8K6F5; B2RN46; D3DSR0
• Molecular Weight: 111,249 Da
• NCBI Official Full Name: bone morphogenetic protein 1 isoform 3
• NCBI Official Synonym Full Names: bone morphogenetic protein 1
• NCBI Official Symbol: BMP1
• NCBI Official Synonym Symbols: PCP; TLD; OI13; PCP2; PCOLC
• NCBI Protein Information: bone morphogenetic protein 1; procollagen C-proteinase; mammalian tolloid protein; procollagen C-endopeptidase
• UniProt Protein Name: Bone morphogenetic protein 1
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP1
• UniProt Synonym Gene Names: PCOLC; BMP-1; mTld; PCP
• UniProt Entry Name: BMP1_HUMAN

NCBI Description

This gene encodes a protein that is capable of inducing formation of cartilage in vivo. Although other bone morphogenetic proteins are members of the TGF-beta superfamily, this gene encodes a protein that is not closely related to other known growth factors. This gene is expressed as alternatively spliced variants that share an N-terminal protease domain but differ in their C-terminal region. [provided by RefSeq, Aug 2008]

Uniprot Description

BMP1: Cleaves the C-terminal propeptides of procollagen I, II and III. Induces cartilage and bone formation. May participate in dorsoventral patterning during early development by cleaving chordin (CHRD). Defects in BMP1 are a cause of autosomal recessive osteogenesis imperfecta (AR-OI). A connective tissue disorder characterized by bone fragility, progressively deforming bones, bowing of limbs due to multiple fractures, very short stature, a triangular face, severe scoliosis, and grayish sclera. AR-OI due to BMP1 mutations belongs to the group of osteogenesis imperfecta type III in the Sillence classification. Belongs to the peptidase M12A family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.19; Cytokine; Protease

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: peptidase activity; growth factor activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; cytokine activity; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; ossification; multicellular organismal development; lipoprotein metabolic process; cell differentiation; proteolysis; skeletal development; cartilage condensation

Disease: Osteogenesis Imperfecta, Type Xiii